Method for electroporation for the early chick embryo
Article first published online: 29 MAY 2008
© 2008 The Authors. Journal compilation © 2008 Japanese Society of Developmental Biologists
Development, Growth & Differentiation
Special Issue: Innovative techniques for the study of development
Volume 50, Issue 6, pages 449–452, August 2008
How to Cite
Hatakeyama, J. and Shimamura, K. (2008), Method for electroporation for the early chick embryo. Development, Growth & Differentiation, 50: 449–452. doi: 10.1111/j.1440-169X.2008.01040.x
- Issue published online: 4 JUL 2008
- Article first published online: 29 MAY 2008
- Received 29 January 2008; revised 06 March 2008; accepted 11 March 2008.
- chick gastrula;
- New culture;
In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures, and some experimental examples are presented.