These authors contributed equally to this work.
Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana
Article first published online: 15 OCT 2009
© 2009 The Authors. Journal compilation © 2009 Japanese Society of Developmental Biologists
Development, Growth & Differentiation
Volume 51, Issue 9, pages 769–775, December 2009
How to Cite
Deguchi, T., Itoh, M., Urawa, H., Matsumoto, T., Nakayama, S., Kawasaki, T., Kitano, T., Oda, S., Mitani, H., Takahashi, T., Todo, T., Sato, J., Okada, K., Hatta, K., Yuba, S. and Kamei, Y. (2009), Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana. Development, Growth & Differentiation, 51: 769–775. doi: 10.1111/j.1440-169X.2009.01135.x
- Issue published online: 26 NOV 2009
- Article first published online: 15 OCT 2009
- Received 22 July 2009; revised 30 August 2009; accepted 31 August 2009.
- gene expression;
- heat shock;
Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.