Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana

Authors

  • Tomonori Deguchi,

    1. Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakouji, Amagasaki, Hyogo 661-0974
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      These authors contributed equally to this work.

  • Mariko Itoh,

    1. Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako-gun, Hyogo 678-1297
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    • #

      These authors contributed equally to this work.

  • Hiroko Urawa,

    1. Laboratory of Plant Organ Development, National Institute for Basic Biology (NIBB), Myodaiji-cho, Okazaki 444-8585
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    • #

      These authors contributed equally to this work.

  • Tomohiro Matsumoto,

    1. Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakouji, Amagasaki, Hyogo 661-0974
    2. First Department of Oral and Maxillofacial Surgery, Tsurumi University, School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Kanagawa 230-0063
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  • Sohei Nakayama,

    1. Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako-gun, Hyogo 678-1297
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  • Takashi Kawasaki,

    1. Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakouji, Amagasaki, Hyogo 661-0974
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  • Takeshi Kitano,

    1. Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555
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  • Shoji Oda,

    1. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa-no-ha 5-1-5, Kashiwa, Chiba 277-8562
    2. Space Biomedical Research Office, Japan Aerospace Exploration Agency (JAXA), Tsukuba Space Center 2-1-1, Sengen, Tsukuba, Ibaraki 305-8505
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  • Hiroshi Mitani,

    1. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa-no-ha 5-1-5, Kashiwa, Chiba 277-8562
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  • Taku Takahashi,

    1. Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530
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  • Takeshi Todo,

    1. Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan
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  • Junichi Sato,

    1. First Department of Oral and Maxillofacial Surgery, Tsurumi University, School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Kanagawa 230-0063
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  • Kiyotaka Okada,

    1. Laboratory of Plant Organ Development, National Institute for Basic Biology (NIBB), Myodaiji-cho, Okazaki 444-8585
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  • Kohei Hatta,

    1. Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako-gun, Hyogo 678-1297
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  • Shunsuke Yuba,

    1. Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-46 Nakouji, Amagasaki, Hyogo 661-0974
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  • Yasuhiro Kamei

    Corresponding author
    1. Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan
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    • Present address: Graduate School of Bioscience, Nagahama Institute of Bio-Science and Technology, Tamura-cho 1266, Nagahama, Shiga 526-0829, Japan.
      Correspondence regarding medaka to S. Yuba, yuba-sns@aist.go.jp; regarding zebrafish to K. Hatta, khatta@sci.u-hyogo.ac.jp; regarding Arabidopsis to H. Urawa, urawaru@nibb.ac.jp.


*Author to whom all correspondence should be addressed.
Email: ykamei@radbio.med.osaka-u.ac.jp

Abstract

Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.

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