Gene manipulation of chick embryos in vitro, early chick culture, and long survival in transplanted eggs

Authors

  • Jun Tanaka,

    1. Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai 980-8575, Japan
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  • Hidekiyo Harada,

    1. Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai 980-8575, Japan
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    • Present address: Kate Storey’s Lab, Division of Cell & Developmental Biology, College of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.

  • Kodai Ito,

    1. Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai 980-8575, Japan
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  • Toshihiko Ogura,

    1. Department of Developmental Neurobiology, Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai 980-8575, Japan
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  • Harukazu Nakamura

    Corresponding author
    1. Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Aoba-ku, Sendai 980-8575, Japan
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  • JT and KI are Research Assistants of Tohoku Neuroscience Global Center of Excellence, HH was a long term fellow of Japan Society for the Promotion of Science.

Author to whom all correspondence should be addressed.
Email: nakamura@idac.tohoku.ac.jp

Abstract

We introduce a revolutionary gene transfer system in chick: transfect chick embryos at early developmental stage by electroporation in vitro, Early Chick (EC) culture, and transplant to the egg to let the embryo survive until E5.5. Referring to the fate map, we could target the tissues of transfection, or transfect large areas of the embryo. We could get tissue-specific expression of a transgene by tissue-specific promoter. This method is very convenient and rapid, but allows us to get stable expression of the transgene in combination with transposon system.

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