Role of syndecan-4 side chains in turkey satellite cell growth and development
Article first published online: 25 JAN 2011
© 2011 The Authors. Journal compilation © 2011 Japanese Society of Developmental Biologists
Development, Growth & Differentiation
Volume 53, Issue 1, pages 97–109, January 2011
How to Cite
Song, Y., McFarland, D. C. and Velleman, S. G. (2011), Role of syndecan-4 side chains in turkey satellite cell growth and development. Development, Growth & Differentiation, 53: 97–109. doi: 10.1111/j.1440-169X.2010.01230.x
- Issue published online: 25 JAN 2011
- Article first published online: 25 JAN 2011
- Received 27 July 2010; revised 26 October 2010; accepted 26 October 2010.
- N-glycosylated chain;
- satellite cell;
Syndecan-4 is a cell membrane heparan sulfate proteoglycan that is composed of a core protein and covalently attached glycosaminoglycans (GAG) and N-linked glycosylated (N-glycosylated) chains. Syndecan-4 has been shown to function independent of its GAG chains. Syndecan-4 may derive its biological function from the N-glycosylated chains due to the biological role of N-glycosylated chains in protein folding and cell membrane localization. The objective of the current study was to investigate the role of syndecan-4 N-glycosylated chains and the interaction between GAG and N-glycosylated chains in turkey myogenic satellite cell proliferation, differentiation, and fibroblast growth factor 2 (FGF2) responsiveness. The wild type turkey syndecan-4 and the syndecan-4 without GAG chains were cloned into the expression vector pCMS-EGFP and used as templates to generate syndecan-4 N-glycosylated one-chain and no-chain mutants with or without GAG chains. The wild type syndecan-4, all of the syndecan-4 N-glycosylated chain mutants were transfected into turkey myogenic satellite cells. Cell proliferation, differentiation, and responsiveness to FGF2 were measured. The overexpression of syndecan-4 N-glycosylated mutants with or without GAG chains did not change cell proliferation, differentiation, and responsiveness to FGF2 compared to the wild type syndecan-4 except that the overexpression of syndecan-4 N-glycosylated mutants without GAG chains increased cell proliferation at 48 and 72 h post-transfection. These data suggest that syndecan-4 functions in an FGF2-independent manner, and the N-glycosylated and GAG chains are required for syndecan-4 to regulate turkey myogenic satellite cell proliferation, but not differentiation.