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dgd1368-sup-0001-FigureS1.pdfapplication/PDF100KFig. S1. The sequences of the LINE-1 partial ORF1 (Clone # 7–10) and ORF2 (Clone # 6–5) cDNAs as well as the genomic DNA sequence of one LINE-1 allele (Clone #18). These DNA sequences were used for the screening of potential LINE-1 piRNAs (Fig. 4).
dgd1368-sup-0002-FigureS2.pdfapplication/PDF100KFig. S2. Genomic DNA polymerase chain reaction (PCR) to obtain the flanking DNA sequences of a putative LINE-1 ORF-2 piRNA listed in Fig. 4. The sequences of the primers used and the 3 clones isolated from the PCR products are shown here. The alignment of these 3 ORF-2 homologous clones and the corresponding DNA fragment in LINE-1 ORF-2 cDNA (Clone #6-5, Supplemental Fig. S1) revealed the polymorphic nature of genomic LINE-1 loci.
dgd1368-sup-0003-FigureS3.pdfapplication/PDF100KFig. S3. The information of Table 1 was extracted from the work by Monaghan JR, et al. (2009). Microarray and cDNA sequence analysis of transcription during nerve-dependent limb regeneration. BMC Biol 7:1. A list of differentially expressed transposon elements (TEs) other than LINE-1. These contigs have been identified by Roche 454 cDNA sequencing in innervated (NR) and denervated (DL) forelimbs after 0 (C0), 5 (NR5 or DL5), and 14 (NR14 or DL14) days post amputation. Listed in Table 2 are the sequences similar to other transposable elements that have also been identified in the same axolotl limb regenerate transcriptome Roche 454 sequencing.

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