These authors contributed equally to this work.
TGF-β3 induces ectopic mineralization in fetal mouse dental pulp during tooth germ development
Version of Record online: 11 APR 2005
Development, Growth & Differentiation
Volume 47, Issue 3, pages 141–152, April 2005
How to Cite
Huojia, M., Muraoka, N., Yoshizaki, K., Fukumoto, S., Nakashima, M., Akamine, A., Nonaka, K. and Ohishi, M. (2005), TGF-β3 induces ectopic mineralization in fetal mouse dental pulp during tooth germ development. Development, Growth & Differentiation, 47: 141–152. doi: 10.1111/j.1440-169x.2005.00790.x
- Issue online: 11 APR 2005
- Version of Record online: 11 APR 2005
- Received 14 October 2004; revised 20 December 2004; accepted 4 January 2005.
- ectopic mineralization;
- pulp cells;
Several members of the transforming growth factor (TGF)-β superfamily are expressed in developing teeth from the initiation stage through adulthood. Of those, TGF-β1 regulates odontoblast differentiation and dentin extracellular matrix synthesis. However, the molecular mechanism of TGF-β3 in dental pulp cells is not clearly understood. In the present study, beads soaked with human recombinant TGF-β3 induced ectopic mineralization in dental pulp from fetal mouse tooth germ samples, which increased in a dose-dependent manner. Further, TGF-β3 promoted mRNA expression, and increased protein levels of osteocalcin (OCN) and type I collagen (COL I) in dental pulp cells. We also observed that the expression of dentin sialophosphoprotein and dentin matrix protein 1 was induced by TGF-β3 in primary cultured dental pulp cells, however, not in calvaria osteoblasts, whereas OCN, osteopontin and osteonectin expression was increased after treatment with TGF-β3 in both dental pulp cells and calvaria osteoblasts. Dentin sialoprotein was also partially detected in the vicinity of TGF-β3 soaked beads in vivo. These results indicate for the first time that TGF-β3 induces ectopic mineralization through upregulation of OCN and COL I expression in dental pulp cells, and may regulate the differentiation of dental pulp stem cells to odontoblasts.