Genotypic subtyping of hepatitis C virus
Article first published online: 10 MAR 2008
Journal of Gastroenterology and Hepatology
Volume 8, Issue 2, pages 150–156, April 1993
How to Cite
CHAYAMA, K., TSUBOTA, A., ARASE, Y., SAITOH, S., KOIDA, I., IKEDA, K., MATSUMOTO, T., KOBAYASHI, M., IWASAKI, S., KOYAMA, S., MORINAGA, T. and KUMADA, H. (1993), Genotypic subtyping of hepatitis C virus. Journal of Gastroenterology and Hepatology, 8: 150–156. doi: 10.1111/j.1440-1746.1993.tb01507.x
- Issue published online: 10 MAR 2008
- Article first published online: 10 MAR 2008
- Accepted for publication 15 October 1992.
- mixed primer set;
- nucleotide sequences;
- polymerase chain reaction
Four subtypes of hepatitis C virus (HCV), Pt(I), K1(II), K2a(III) and K2b(IV), have been suggested based on the nucleotide sequences of the non-structural (NS) 5 region. A fifth subtype from Japanese patients, Tr(V), which shows a less than 68% homology in nucleotide sequence when compared with other subtypes has been identified. A one-step method which enables a quick determination of subtype using polymerase chain reaction with a mixed primer set deduced from the sequence of each subtype has been developed. Using this technique, the subtypes of 418 out of 478 Japanese patients (87.4%) were determined. The incidence of each subtype in Japan was as follows: K1(II), 307 (73.4%); K2a(III), 74 (17.7%); K2b(IV), 28 (6.7%); and Tr(V), 3 (0.7%). This one-step subtyping technique should be useful for studying the epidemiology or biology of the HCV.