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Autoantibodies against a 210kDa glycoprotein of the nuclear pore complex as a prognostic marker in patients with primary biliary cirrhosis

Authors

  • SHINICHI ITOH,

    1. Third Department of Internal Medicine, Niigata University School of Medicine and , College of Biomedical Technology, Niigata University, Niigata City, Japan
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  • TAKAFUMI ICHIDA,

    1. Third Department of Internal Medicine, Niigata University School of Medicine and , College of Biomedical Technology, Niigata University, Niigata City, Japan
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  • TOSHIAKI YOSHIDA,

    1. Third Department of Internal Medicine, Niigata University School of Medicine and , College of Biomedical Technology, Niigata University, Niigata City, Japan
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  • AKIHITO HAYAKAWA,

    1. Third Department of Internal Medicine, Niigata University School of Medicine and , College of Biomedical Technology, Niigata University, Niigata City, Japan
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  • MORIAKI UCHIDA,

    1. Third Department of Internal Medicine, Niigata University School of Medicine and , College of Biomedical Technology, Niigata University, Niigata City, Japan
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  • TOMOKO TASHIRO-ITOH,

    1. Third Department of Internal Medicine, Niigata University School of Medicine and , College of Biomedical Technology, Niigata University, Niigata City, Japan
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  • YASUNOBU MATSUDA,

    1. Third Department of Internal Medicine, Niigata University School of Medicine and , College of Biomedical Technology, Niigata University, Niigata City, Japan
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  • KIYOSHI ISHIHARA,

    1. Third Department of Internal Medicine, Niigata University School of Medicine and , College of Biomedical Technology, Niigata University, Niigata City, Japan
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  • HITOSHI ASAKURA

    1. Third Department of Internal Medicine, Niigata University School of Medicine and , College of Biomedical Technology, Niigata University, Niigata City, Japan
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Dr Takafumi Ichida Third Department of Internal Medicine, Niigata University School of Medicine, 757 Asahimachi-Dori 1, Niigata City 951, Japan. Email: <takafumi@med.niigata-u.ac.jp>

Abstract

It has been reported that the presence of anti-nuclear antibody against a 210kDa glycoprotein of nuclear pore complex (anti-gp210) is highly specific for primary biliary cirrhosis (PBC). The aim of the present study was to investigate the significance of anti-gp210, especially as a prognostic marker. The presence of anti-gp210 was ascertained in 113 patients with PBC and 162 controls by indirect immunofluorescence assay using HepG2 cells and immunoblotting analysis using nuclear extracts from HeLa cells. Anti-gp210 was detected in 25 of the 113 (22.1%) patients. None of the 162 controls was positive for anti-gp210. The appearance and titre of anti-gp210 in the patients with PBC did not vary from the time of diagnosis and through their clinical course. Anti-mitochondrial antibodies (AMA), including antibodies against pyruvate dehydrogenase complex, branched chain α-ketoacid dehydrogenase complex and α-ketoglutarate dehydrogenase complex, were not detected by enzyme-linked immunosorbent assay in five of the 113 (4.4%) patients with PBC. However, anti-gp210 alone was positive in one of these five patients. The difference in prognosis was statistically significant; patients with PBC positive for anti-gp210 died from hepatic failure more frequently than those who were negative (P < 0.01), although there were no statistically significant differences in the frequency of jaundice and the histological stage at the time of diagnosis between the two groups. We suggest that the presence of anti-gp210 is one of the independent prognostic markers able to predict, at the time of diagnosis, a poor outcome in patients with PBC.

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