Present address: Department of Gastroenterology and Hepatology, Yanbian University School of Medicine, Yanji, Jilin 133000, China.
417–2α-Tocopherol and ascorbic acid attenuates the ribavirin-induced decrease of eicosapentaenoic acid in erythrocyte membrane in chronic hepatitis C patients
Version of Record online: 21 APR 2006
Journal of Gastroenterology and Hepatology
Volume 21, Issue 8, pages 1269–1275, August 2006
How to Cite
Hino, K., Murakami, Y., Nagai, A., Kitase, A., Hara, Y., Furutani, T., Ren, F., Yamaguchi, Y., Yutoku, K., Yamashita, S., Okuda, M., Okita, M. and Okita, K. (2006), 417–2α-Tocopherol and ascorbic acid attenuates the ribavirin-induced decrease of eicosapentaenoic acid in erythrocyte membrane in chronic hepatitis C patients. Journal of Gastroenterology and Hepatology, 21: 1269–1275. doi: 10.1111/j.1440-1746.2006.04378.x
- Issue online: 10 JUL 2006
- Version of Record online: 21 APR 2006
- Accepted for publication 24 August 2005.
Vol. 21, Issue 10, 1640, Version of Record online: 18 AUG 2006
- fatty acid;
- hemolytic anemia;
- lipid peroxidation;
- oxidative stress
Background: Oxidative damage of the erythrocyte membrane plays an important role in ribavirin-induced anemia. The purpose of the present paper was to assess whether supplementation of α-tocopherol and ascorbic acid (vitamins) causes changes in the erythrocyte membrane fatty acid composition during interferon and ribavirin combination therapy for chronic hepatitis C patients.
Methods: Fatty acid compositions in erythrocyte membrane phospholipids were determined by gas chromatography at 0, 2, 4, 8 weeks, and at the end of combination therapy (26 weeks) for interferon with ribavirin in 32 patients with chronic hepatitis C who were randomized to receive vitamins or not (controls).
Results: Good compliance with orally administered vitamins and ribavirin were confirmed by their concentrations in erythrocytes or plasma. The hemoglobin level was negatively correlated with the ribavirin concentration at 8 weeks (r = 0.59, P = 0.01) after initiation of therapy in controls, but not in the vitamin group. Among the 26 kinds of fatty acids analyzed, only eicosapentaenoic acid (EPA) significantly decreased at 8 weeks after initiation of therapy (P = 0.03) and at the end of therapy (P = 0.004) in controls. Vitamins did not inhibit ribavirin-induced anemia, but attenuated the decrease of EPA in erythrocytes. The EPA level was negatively correlated with the drop in hemoglobin levels at 8 weeks after initiation of therapy in controls (r = 0.58, P = 0.015), but not in the vitamin group.
Conclusions: Supplementation of α-tocopherol and ascorbic acid attenuates the ribavirin-induced decrease of EPA in erythrocyte membrane phospholipids in chronic hepatitis C patients.