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Keywords:

  • antigen presentation;
  • hepatocellular carcinoma;
  • human leukocyte antigens;
  • immunoproteasome;
  • interferon-γ

Abstract

Background:  Tumor cells may alter the expression of numerous components involved in antigen-processing machinery to decrease human leukocyte antigen (HLA) class I expression, allowing the tumor cells to escape immune surveillance. The purpose of the present study was to investigate the involvement of these components in the downregulation of HLA class I expression in human hepatocellular carcinoma cell line BEL7404.

Methods:  Expression of HLA-I and antigen presentation-related genes were analyzed by flow cytometry and polymerase chain reaction. The HLA class I-deficient BEL7404 cell was transfected with the low-molecular-weight protein (LMP) 2 and LMP7 gene and were analyzed by flow cytometry for restoration of surface HLA class I expression.

Results:  The BEL7404 cells downregulated the expression of HLA class I antigen and lacked expression of LMP2 and LMP7. Interferon (IFN)-γ treatment increased the expression of LMP2 but not LMP7. The LMP2-transfected BEL7404 cells or LMP2 and LMP7-cotransfected cells restored surface HLA class I expression while LMP7-transfected cells did not. However, in IFN-γ-treated BEL7404 cells, transfection with the LMP7 gene induced more HLA class I expression than mock transfection.

Conclusions:  The LMP2 gene was required for the expression of HLA class I molecules in BEL7404. The LMP7 was not the major reason for loss of HLA class I in BEL7404 cells, although the supply of exogenous LMP7 could increase surface expression of HLA class I antigen.