Clinicopathological features of hepatocellular carcinoma evaluated by vascular endothelial growth factor expression
Version of Record online: 19 APR 2007
Journal of Gastroenterology and Hepatology
Volume 22, Issue 12, pages 2202–2207, December 2007
How to Cite
Amaoka, N., Osada, S., Kanematsu, M., Imai, H., Tomita, H., Tokuyama, Y., Sakashita, F., Nonaka, K., Goshima, S., Kondo, H. and Adachi, Y. (2007), Clinicopathological features of hepatocellular carcinoma evaluated by vascular endothelial growth factor expression. Journal of Gastroenterology and Hepatology, 22: 2202–2207. doi: 10.1111/j.1440-1746.2006.04790.x
- Issue online: 19 NOV 2007
- Version of Record online: 19 APR 2007
- Accepted for publication 31 August 2006.
- hepatocellular carcinoma;
- liver condition;
- vascular endothelial growth factor;
- western blot analysis
Aim: To evaluate the significance of the expression of vascular endothelial growth factor (VEGF), its correlation with clinicopathological variables were studied in the tissue of hepatocellular carcinoma (HCC) and surrounding liver.
Methods: In 56 samples (tumor and non-tumor liver tissue) collected from 28 patients, VEGF expression was examined by immunohistochemistry and western blot analysis.
Results: The value of VEGF expression by western blotting was correlated with immunohistochemical staining grade. In tumor tissue, the value of VEGF expression correlated with tumor size (P = 0.034), á-fetoprotein (P = 0.036) and protein induced by vitamin K absence-II by simple regression, and histological grade (P = 0.0132) by the unpaired t-test. The level of VEGF expression in non-tumor liver was found to correlate with the value of serum albumin (P = 0.008), cholinesterase (P = 0.012) and prothrombin activity (P = 0.046). The frequency of simple nodular type in gross appearance decreased in cases with high tumor/non-tumor (T/N) ratio (P = 0.022), and the degree of portal vein invasion progressed with an increase in the T/N ratio (P = 0.008). The T/N ratio was significantly higher in early recurrence cases (P = 0.0081).
Conclusion: This study on the expression of VEGF might be useful to estimate the liver condition and the clinicopathological features of HCC.