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Keywords:

  • gastric cancer;
  • haplotype;
  • polymorphism;
  • TGF-β1 gene

Abstract

Background and Aim:  As an important cytokine that modulate the cell cycle, the involvement of transforming growth factor beta-1 (TGF-β1) in carcinogenesis has been extensively studied for many years. Literatures have demonstrated that TGF-β1 gene polymorphisms may alter the risk of various cancers, such as lung, prostate and breast. To investigate whether polymorphisms of the TGF-β1 gene can modify the risk of gastric cancer, we conduct this hospital-based, case-control study.

Methods:  One hundred and sixty-seven cases and 193 gender, age-matched healthy controls were enrolled in this case-control study. TGF-β1 polymorphisms C-509T and T + 869C were identified by PCR-RFLP and ARMS-PCR protocols, respectively.

Results:  Significantly different distributions of both genes were demonstrated between the case and control. Variant genotypes −509CT, −509TT, +869TC and +869CC were associated with increased risk of gastric cancer (P = 0.001, OR = 2.54; P = 0.016, OR = 2.09; P < 0.001, OR = 3.46; P < 0.001, OR = 4.04, respectively). With haplotype analysis, wild type CT (−509C and +869T) led to a lower frequency in case than that in control (P < 0.001), while haplotype TC was more frequent in case than in control (P < 0.001). Multiple logistic regression analysis revealed that individuals with haplotype TC had an increased likelihood of developing gastric cancer (OR = 3.19, 95%CI = 1.72–5.90).

Conclusions:  Our findings imply that −509C > T and +869T > C gene polymorphisms in TGF-β1 may be a critical risk factor of genetic susceptibility to gastric cancer in the Chinese population.