Identification of a novel protein binding to hepatitis C virus core protein
Article first published online: 13 APR 2009
© 2009 The Authors. Journal compilation © 2009 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd
Journal of Gastroenterology and Hepatology
Volume 24, Issue 7, pages 1300–1304, July 2009
How to Cite
Chen, Y.-r., Chen, T.-y., Zhang, S.-l., Lin, S.-m., Zhao, Y.-r., Ye, F., Zhang, X., Shi, L., Dang, S.-s. and Liu, M. (2009), Identification of a novel protein binding to hepatitis C virus core protein. Journal of Gastroenterology and Hepatology, 24: 1300–1304. doi: 10.1111/j.1440-1746.2009.05846.x
- Issue published online: 29 JUL 2009
- Article first published online: 13 APR 2009
- Accepted for publication 3 February 2009.
- core protein;
- protein-protein interaction;
- yeast two-hybrid
Background: Hepatitis C virus (HCV) core protein is a multi-functional viral protein that interacts with several target proteins of both viral and cellular origin.
Aim and Methods: To gain insight into the mechanism of action of HCV core protein, we used a yeast two-hybrid system to identify the core protein-interacting cellular targets.
Results: A cDNA clone encoding an aspartoacylase was obtained, termed aspartoacylase 3 (ACY3). Interaction between ACY3 and HCV core protein was verified using a co-immunoprecipitation assay in vitro, and a mammalian two-hybrid system in vivo. Fluorescence microscopy showed green fluorescence protein-fused ACY3 localized in the cytoplasm.
Conclusion: Our data suggest that ACY3 is an HCV core binding protein, which may play a role in the development of HCV-associated diseases.