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Endoplasmic reticulum stress involved in the course of lipogenesis in fatty acids-induced hepatic steatosis

Authors

  • Jiang Liu,

    1. Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
    2. Department of Gastroenterology, Huzhou Central Hospital, Huzhou 313000, Zhejiang Province, China
      (Correction made after online publication 15 December 2009: Amendment of author affiliation for Jiang Liu and Wei-Ping Li)
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  • Xi Jin,

    1. Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
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  • Chao-Hui Yu,

    1. Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
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  • Shao-Hua Chen,

    1. Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
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  • Wei-Ping Li,

    1. Department of Gastroenterology, Huzhou Central Hospital, Huzhou 313000, Zhejiang Province, China
      (Correction made after online publication 15 December 2009: Amendment of author affiliation for Jiang Liu and Wei-Ping Li)
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  • You-Ming Li

    Corresponding author
    1. Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China
    • You-Ming Li, Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China. Email: liujiangxm@yahoo.com.cn

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Abstract

Background and Aims:  The aim of the present study is to elucidate whether endoplasmic reticulum stress involved in the course of lipogenesis in fatty acids induced hepatic steatosis and the potential effect of metformin on endoplasmic reticulum stress.

Methods:  HepG2 cells were exposed to different types of culture media. After incubation for 24 h, cells were harvested to evaluate cell survival rate and lipid level among different groups. Moreover, reverse transcriptase polymerase chain reaction and western blot for glucose-regulated protein-78 (GRP78), sterol response element-binding protein-1c (SREBP1c) and fatty acid synthase (FAS) were applied.

Results:  The levels of triglyceride (TG), mRNA of FAS, mRNA and protein of GRP78 and SREBP1c significantly increased in the free fatty acids (FFA)-induced hepatic steatosis group. Then, HepG2 cells with hepatic steatosis induced by FFA were treated by metformin, levels of TG, GRP78 mRNA, SREBP1c mRNA and FAS mRNA as well as GRP78 and SREBP1 protein levels were partially decreased but without significant differences.

Conclusion:  Endoplasmic reticulum stress might be involved in lipogenesis in fatty acids-induced hepatic steatosis. Therefore, endoplasmic reticulum stress might serve as a novel target in the pathogenesis and therapy of non- alcoholic fatty liver disease.

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