• Hepatitis B virus envelope protein;
  • Fructose-1,6-bisphosphate aldolase type A;
  • Mammalian two-hybrid;
  • Protein interaction


Hepatitis B virus (HBV) infection poses great challenge to humans, claiming 1 million lives annually worldwide. Solid data have related HBV to hepatocellular carcinoma. In the present research, we verified the interaction between surface protein (HBs) encoded by HBV and aldolase A (ALDA) using yeast two-hybrid, mammalian two-hybrid, co-immunoprecipitation, GST pull-down and laser scanning confocal. Then we further determined that S region within HBs is sufficient for binding ALDA. In addition, ALDA conferred protection to ultraviolet radiation-induced apoptosis, and this effect was enhanced by the interaction between HBs and ALDA. Results of the present research would help in the exploration of novel roles of ALDA in the oncogenesis of the liver.