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Supplement 1 The representative triple-label fluorescence immunohistochemistry image of HSC after small-for-size liver transplantation. (A) HSC labeled with rabbit polyclonal to ETAR and secondary antibodies conjugated to fluorescein isothiocyanate (FITC). Under the excitation wave of 488 nm, the tissue was visualized in green. (B) HSC labeled with mouse monoclonal to GFAP and secondary antibodies conjugated to tetramethylrhodamine isothiocyanate (TRITC). Under the excitation wave of 633 nm, the tissue was visualized in red. (C) Slides were incubation with 4′-6-diamidino-2-phenylindole (DAPI) which had affinity with nucleus. Under the excitation wave of 359 nm, the tissue was visualized in blue. (D) The merged image of the (A), (B), and (C) which captured on the same site.

Supplement 2 The representative triple-label fluorescence immunohistochemistry image of HSC after small-for-size liver transplantation. (A) HSC labeled with rabbit polyclonal to ETBR and secondary antibodies conjugated to fluorescein isothiocyanate (FITC). Under the excitation wave of 488 nm, the tissue was visualized in green. (B) HSC labeled with mouse monoclonal to GFAP and secondary antibodies conjugated to tetramethylrhodamine isothiocyanate (TRITC). Under the excitation wave of 633 nm, the tissue was visualized in red. (C) Slides were incubation with 4′-6-diamidino-2-phenylindole (DAPI) which had affinity with nucleus. Under the excitation wave of 359 nm, the tissue was visualized in blue. (D) The merged image of the (A), (B), and (C) which captured on the same site.

Supplement 3 The representative intravital fluorescence microscopic images of sinusoidal perfusion of every group. (A) The normal liver; (B) SLT group at 1 day; (C) SLT-AR group at 1 h; (D) SLT-AR group at 1 day; (E) SLT-BR group at 1 h; (F) SLT-BR group at 1 day. Original magnification ×64. [RIGHTWARDS ARROW], sinusoidal non-perfusion region.

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