GRM3, glutamate receptor, metabotropic 3.
Large-scale case-control study of a functional polymorphism in the glutamate receptor, metabotropic 3 gene in patients with schizophrenia
Article first published online: 14 APR 2008
© 2008 The Authors
Psychiatry and Clinical Neurosciences
Volume 62, Issue 2, pages 239–240, April 2008
How to Cite
Nunokawa, A., Watanabe, Y., Kitamura, H., Kaneko, N., Arinami, T., Ujike, H., Inada, T., Iwata, N., Kunugi, H., Itokawa, M., Ozaki, N. and Someya, T. (2008), Large-scale case-control study of a functional polymorphism in the glutamate receptor, metabotropic 3 gene in patients with schizophrenia. Psychiatry and Clinical Neurosciences, 62: 239–240. doi: 10.1111/j.1440-1819.2008.01762.x
- Issue published online: 14 APR 2008
- Article first published online: 14 APR 2008
- Received 10 May 2007; revised 10 May 2007; accepted 29 October 2007.
THE A ALLELE of rs6465084, a single nucleotide polymorphism (SNP) of the glutamate receptor, metabotropic 3 (GRM3) gene, has been found to be associated with decreased verbal fluency and reduced prefrontal cortical levels of N-acetylaspartate/creatine.1 This functional SNP has been shown to be associated with schizophrenia1 but other studies failed to demonstrate such an association.2–5 To further investigate the inconsistent results, we conducted a case–control association study.
The Ethics Committee on Genetics of each participating institute approved the present study. All participants provided written informed consent. All participants were unrelated Japanese subjects. The subjects consisted of 2358 patients with schizophrenia, meeting the DSM-IV criteria (1273 men, 1085 women; mean age 46.8 ± 14.7 years), and 2433 control subjects (1313 men, 1120 women; mean age 45.6 ± 13.8 years). We genotyped rs6465084 (C_11245618_10; Applied Biosystems, CA, USA) using the TaqMan 5′-exonuclease assay.6 We did not examine rs1468412,7 rs22992258 and rs2746229 because these SNP have not been shown to be associated with schizophrenia in a large Japanese sample,5 which overlapped the subjects in the present study.
The genotype distributions did not deviate significantly from Hardy–Weinberg equilibrium in either group. The genotype and allele frequencies in patients did not differ from those in controls (Table 1). The results of the present study indicate that the rs6465084 functional polymorphism in GRM3 does not contribute to genetic susceptibility to schizophrenia.
|Patients (%)||Controls (%)||P|
|A/A||2045 (86.7)||2132 (87.6)|
|A/G||305 (12.9)||289 (11.9)|
|G/G||8 (0.4)||12 (0.5)|
|A||4395 (93.2)||4553 (93.6)|
|G||321 (6.8)||313 (6.4)|
- 5Replication study and meta-analysis of the genetic association of GRM3 gene polymorphisms with schizophrenia in a large Japanese case-control population. Am. J. Med. Genet. B Neuropsychiatr. Genet. 2007 Oct 19 [Epub ahead of print]., , et al.