Cooperative interactions among p53, bcl-2 and Epstein–Barr virus latent membrane protein 1 in nasopharyngeal carcinoma cells
Article first published online: 4 JUN 2004
Volume 54, Issue 7, pages 475–485, July 2004
How to Cite
Sheu, L.-F., Chen, A., Lee, H.-S., Hsu, H.-Y. and Yu, D.-S. (2004), Cooperative interactions among p53, bcl-2 and Epstein–Barr virus latent membrane protein 1 in nasopharyngeal carcinoma cells. Pathology International, 54: 475–485. doi: 10.1111/j.1440-1827.2004.01654.x
- Issue published online: 4 JUN 2004
- Article first published online: 4 JUN 2004
- Received 8 January 2004. Accepted for publication 5 March 2004.
- Epstein–Barr virus;
- latent membrane protein 1;
- nasopharyngeal carcinoma;
Interactions among p53, bcl-2 and Epstein–Barr virus (EBV) latent membrane protein 1 (LMP1) in nasopharyngeal carcinoma (NPC) cells were evaluated by gene cotransfections. The data showed that bcl-2 expression was not only able to prevent the growth suppression induced by wild-type p53 but was also paradoxically able to inhibit the growth enhancement induced by mutant p53. Latent membrane protein 1 was shown to be capable of overcoming the growth inhibition induced by wild-type p53 and the synergistic cooperation with bcl-2 to enhance cellular growth. Latent membrane protein 1 could also cooperate with mutant p53 to provide a growth advantage for NPC cells. Most NPC revealed detectable overexpression of p53, and the majority of those were a wild type possibly responding to EBV infection. The coexpression of bcl-2 and LMP1 was thought to inhibit the growth suppression induced by wild-type p53 in NPC. But there was no associated expression between LMP1 and bcl-2 because we demonstrated that transfected LMP1 failed to induce bcl-2 expression in NPC cells in contrast to the findings in B cells. It is theorized that the cooperative expression of bcl-2 and LMP1 exists in the majority of NPC, while a minority of NPC have cooperative expression of LMP1 and mutant p53. Each cooperative interaction could play an important role in the development and progression of NPC.