Communicating editor: K. Ishida.
Latitudinal and intracolony ITS-rDNA sequence variation in the symbiotic dinoflagellate genus Symbiodinium (Dinophyceae) in Zoanthus sansibaricus (Anthozoa: Hexacorallia)
Version of Record online: 19 JUL 2006
Volume 54, Issue 2, pages 122–132, June 2006
How to Cite
Reimer, J. D., Takishita, K., Ono, S., Maruyama, T. and Tsukahara, J. (2006), Latitudinal and intracolony ITS-rDNA sequence variation in the symbiotic dinoflagellate genus Symbiodinium (Dinophyceae) in Zoanthus sansibaricus (Anthozoa: Hexacorallia). Phycological Research, 54: 122–132. doi: 10.1111/j.1440-1835.2006.00419.x
- Issue online: 19 JUL 2006
- Version of Record online: 19 JUL 2006
- Received 23 May 2005; accepted 18 November 2005.
We sequenced the internal transcribed spacer of ribosomal DNA (ITS-rDNA) of Symbiodinium spp. (Freudenthal) from conspecific Zoanthus sansibaricus (Carlgren) colonies along a latitudinal gradient in Japan. Phylogenetic analysis reveals that Zoanthus in the two northern sites of Kokubu and Sakurajima harbor exclusively Symbiodinium subclade C1, whereas Yakushima Zoanthus harbors Symbiodinium subclades C1 and C15, and southernmost Amami Zoanthus Symbiodinium subclades A1 and C1, indicating holobiont flexibility. Individual Zoanthus colonies associated exclusively with one single subclade, but unexpectedly there was small variation between Symbiodinium ITS-rDNA clone sequences obtained from within individual Zoanthus colonies. There was also a large deletion in the ITS-2/28S rDNA boundary region in one clone sequence, and another large deletion in the 5.8S rDNA region in another clone. Our intracolony sequence heterogeneity might be a result of the presence of multiple copies of the ITS-rDNA region within individual Symbiodinium genomes, or result from the possible presence of closely related Symbiodinium genotypes in the host.