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Keywords:

  • genetic resource;
  • polymerase chain reaction-restriction fragment length polymorphism;
  • Porphyra yezoensis;
  • Porphyra tenera;
  • rbcL;
  • wild species

SUMMARY

To discriminate between cultivated Porphyra species (Porphyra yezoensis and Porphyra tenera) and closely related wild Porphyra species, we developed a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the rbcL gene using five restriction enzymes. Although our previous PCR-RFLP analyses of internal transcribed spacer (ITS) rDNA and plastid RuBisCO spacer regions could not always discriminate wild P. yezoensis, wild P. tenera, and closely related wild species, the PCR-RFLP profiles of the rbcL gene were useful in discriminating samples collected from natural habitats. Therefore, PCR-RFLP analysis of the rbcL gene will help in the simple identification of a large number of samples, not only for the establishment of reliable cultures as breeding material, but also for the taxonomic investigations of species that are closely related to cultivated Porphyra.