Communicating editor: U. Karsten.
Sterol biosynthesis in the harmful marine dinoflagellate, Karenia brevis: Identification of biosynthetic intermediates produced during exposure to the fungicide fenpropidine
Version of Record online: 28 DEC 2010
© 2010 Japanese Society of Phycology
Volume 59, Issue 1, pages 54–63, January 2011
How to Cite
Leblond, J. D., Roche, S. A., Porter, N. M., Howard, J. C. and Dunlap, N. K. (2011), Sterol biosynthesis in the harmful marine dinoflagellate, Karenia brevis: Identification of biosynthetic intermediates produced during exposure to the fungicide fenpropidine. Phycological Research, 59: 54–63. doi: 10.1111/j.1440-1835.2010.00598.x
- Issue online: 28 DEC 2010
- Version of Record online: 28 DEC 2010
- Received 4 June 2010; accepted 27 September 2010.
Karenia brevis is a harmful marine dinoflagellate that forms yearly blooms in the Gulf of Mexico. Under normal growth conditions, K. brevis forms two predominant sterols (24R)-4α-methyl-5α-ergosta-8(14),22-dien-3β-ol (gymnodinosterol) and its 27-nor isomer (brevesterol). At the current time, there are no published studies concerning the biosynthesis of these two sterols. We have therefore undertaken experiments in which K. brevis was exposed to the fungicide fenpropidine, an inhibitor of the Δ14-reductase and the Δ87-isomerase that operate in sterol biosynthesis in both fungal and plant systems. Such exposure to fenpropidine has produced two, tri-unsaturated intermediates. The identifications of these two K. brevis sterol biosynthesis intermediates, via gas chromatography/mass spectrometry and nuclear magnetic resonance spectroscopy techniques, were 4α-methyl-5α-ergosta-8,14,22-trien-3β-ol and 5α-ergosta-8,14,22-trien-3β-ol.