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Appendix S1. The information of primers to amplify and sequence the ITS region and 18S rRNA gene.

Appendix S2. Modified AQUIL medium for culture experiments using Cladophora strains established from fresh, brackish and seawater, respectively. Each value indicates a final concentration (mol) in the working solution.

Appendix S3. Pairwise distance comparison of sequence variation in Cladophora glomerata and its related species from the aligned ribosomal ITS regions. The lower half of the matrix shows the actual number of base pair changes between sequences. The upper half of the matrix shows the corrected distances between sequences, calculated using maximum composite likelihood model (Tamura et al. 2004).

Appendix S4. Pairwise distance comparison of sequence variation in Cladophora albida and its related species from the aligned ribosomal ITS regions.

Appendix S5. Specimen number of each C. albida ribotype detected in each month.

FilenameFormatSizeDescription
PRE_641_sm_AppendixS1.docx19KSupporting info item
PRE_641_sm_AppendixS2.docx52KSupporting info item
PRE_641_sm_AppendixS3.docx85KSupporting info item
PRE_641_sm_AppendixS4.docx96KSupporting info item
PRE_641_sm_AppendixS5.docx40KSupporting info item

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