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pre658-sup-0001-si.doc6713K

Fig. S1. PCR profiles for the five markers used.

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Fig. S2. Slope of saturation curves for detecting saturation within a marker region.

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Fig. S3. Maximum-likelihood topology (root A) of the combined dataset (matrix-I).

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Fig. S4. Bayesian topology (root B) using the second alignment (matrix-II).

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Fig. S5. Bayesian tree (root C) using a relaxed molecular clock assumption on the ingroup alignment.

pre658-sup-0002-si.doc563K

Table S1. Species identification, sample ID, location with geographic information (if available), and GenBank accession numbers.

pre658-sup-0002-si.doc563K

Table S2. Primer characteristics and their coded PCR-profiles.

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Table S3. Partition Homogeneity Test or Incongruence Length Test.

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Table S4. Estimation of mutation rates using HKY-distances.

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Table S5. Partition strategy test.

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Table S6. HKY-distances between and within lineage.

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Table S7. Molecular clock test.

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Table S8. Topology test.

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Table S9. Average difference in GC-content.

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