Siu P. Ho carried out all the biochemical measurements, Moira Chan-Yeung and Mary S. M. Ip recruited all the subjects, Kathy K. M. Chow carried out all the database entry and statistical analysis and Judith C. W. Mak initiated the study and wrote the manuscript.
Antioxidant enzyme activities in healthy Chinese adults: influence of age, gender and smoking*
Article first published online: 9 JUN 2005
Volume 10, Issue 3, pages 305–309, June 2005
How to Cite
HO, S. P., CHAN-YEUNG, M., CHOW, K. K.M., IP, M. S.M. and MAK, J. C.W. (2005), Antioxidant enzyme activities in healthy Chinese adults: influence of age, gender and smoking. Respirology, 10: 305–309. doi: 10.1111/j.1440-1843.2005.00702.x
- Issue published online: 9 JUN 2005
- Article first published online: 9 JUN 2005
- Received 14 September 2004; accepted for publication 2 December 2004.
- glutathione peroxidase;
- superoxide dismutase
Objective: Specific antioxidant enzymes play a vital role in regulating and maintaining oxidant species. The aim of this study was to determine these antioxidant enzyme activities (i.e. catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx)) in erythrocytes from a group of healthy Chinese subjects and to study the influence of age, gender and smoking habits on the enzyme activities.
Methodology: Chinese healthy subjects (n = 276) were grouped according to their age range, gender differences and smoking habits. Antioxidant enzyme activities were measured spectrophotometrically using standard kinetic methods.
Results: There was a significant decrease in erythrocyte GPx activity in ever-smokers compared with non-smokers (47.10 ± 1.33 mU/g haemoglobin (Hb) vs. 51.41 ± 1.64 mU/g Hb, P < 0.05). Age-related significant increases in erythrocyte CAT and SOD activities were found in non-smokers but not in ever-smokers. There was no age-related difference in erythrocyte GPx activity in either non-smokers or ever-smokers. Among those >60 years old, erythrocyte CAT and GPx activities were significantly lower in ever-smokers than in non-smokers (29.70 ± 3.07 mU/g Hb vs. 41.63 ± 4.92 mU/g Hb (P < 0.05), and 47.55 ± 2.00 mU/g Hb vs. 55.30 ± 3.60 mU/g Hb (P < 0.05), respectively). It was also found that females had higher erythrocyte GPx activity than males but this difference did not reach significance in non-smokers.
Conclusion: From the results of this study, it is concluded that oxidative stress seems to be present in elderly ever-smokers among the Chinese population.