Takahide Nagase, being an Associate Editor for Respirology, did not influence the outcome of this manuscript.
Calcitonin gene-related peptide mediates acid-induced lung injury in mice
Article first published online: 22 OCT 2007
Volume 12, Issue 6, pages 807–813, November 2007
How to Cite
AOKI-NAGASE, T., NAGASE, T., OH-HASHI, Y., KURIHARA, Y., YAMAGUCHI, Y., YAMAMOTO, H., NAGATA, T., KURIHARA, H. and OUCHI, Y. (2007), Calcitonin gene-related peptide mediates acid-induced lung injury in mice. Respirology, 12: 807–813. doi: 10.1111/j.1440-1843.2007.01172.x
- Issue published online: 22 OCT 2007
- Article first published online: 22 OCT 2007
- Received 11 December 2006; invited to revise 23 January 2007; revised 28 January 2007; accepted 5 March 2007 (Associate Editor: Yoichi Nakanishi).
- acid aspiration;
- acute lung injury;
- acute respiratory distress syndrome;
- calcitonin gene-related peptide;
- knockout mice
Background and objective: Acid-induced lung injury from aspiration is one of the most important causes of ARDS. Calcitonin gene-related peptide (CGRP) is a neuropeptide that has various biological actions. The current study investigated whether CGRP might have pathophysiological roles in acid-induced lung injury.
Methods: The investigations employed CGRP gene-disrupted mice––mutant mice (CGRP–/–) and their littermate controls (CGRP+/+). Anaesthetized and mechanically ventilated mice received 2 mL/kg HCl (pH = 1.5) intratracheally. Lung wet-to-dry weight ratios were calculated to assess pulmonary oedema, total and differential cell counts of the BALF were determined, and measurements of myeloperoxidase activity were performed.
Results: Acid-induced lung injury was characterized by an increase in lung permeability and respiratory failure. Disruption of the CGRP gene significantly attenuated acid-induced injury, oedema and respiratory failure.
Conclusions: This study suggests that CGRP is involved in the pathogenesis of acute lung injury caused by acid aspiration and CGRP mutant mice may provide an appropriate model to study molecular mechanisms in this context.