Inflammatory markers in exhaled breath condensate from patients with asthma
Article first published online: 29 MAY 2008
© 2008 The Authors. Journal compilation © 2008 Asian Pacific Society of Respirology
Volume 13, Issue 5, pages 654–663, July 2008
How to Cite
UENO, T., KATAOKA, M., HIRANO, A., IIO, K., TANIMOTO, Y., KANEHIRO, A., OKADA, C., SODA, R., TAKAHASHI, K. and TANIMOTO, M. (2008), Inflammatory markers in exhaled breath condensate from patients with asthma. Respirology, 13: 654–663. doi: 10.1111/j.1440-1843.2008.01315.x
- Issue published online: 10 JUL 2008
- Article first published online: 29 MAY 2008
- Received 4 September 2007; invited to revise 26 October 2007; 26 November 2007; revised 13 November 2007; 3 December 2007; accepted 5 December 2007 (Associate Editor: Takahide Nagase).
- clinical allergy and immunology;
- exhaled breath condensate
Background and objective: Evaluation of airway inflammation is important for the diagnosis and treatment of asthma. Exhaled breath condensate (EBC) is a minimally invasive method for assessing inflammation and may be useful for monitoring airway inflammation in asthma. The aims of this study were to establish an EBC collection method, to assess biomarkers reflecting asthmatic airway inflammation, and to determine the relationship of these biomarkers with asthma severity and lung function.
Methods: Fifty-eight non-smoking healthy subjects, seven asymptomatic smokers, nine subjects with common cold and 55 asthmatics with disease severity ranging from mild intermittent to severe persistent were studied. The efficacy of a pipette method was compared with that of a commercial collecting device. pH, CRP, albumin, hydrogen peroxide (H2O2) and nitrite/nitrate levels were measured in EBC.
Results: Except for the quantity of EBC collected and albumin levels, there were no differences between the commercial method and the pipette method in levels of biomarkers measured. Levels of CRP, H2O2 and nitrite/nitrate were significantly higher in the asthma group than that in the control group. In terms of asthma severity, pH and levels of CRP, H2O2 and nitrate were significantly higher in the mild persistent group than that in the other groups. In addition, H2O2 levels in EBC correlated significantly with the level of nitrite/nitrate. FEV1 and PEF showed significant negative correlations with H2O2 and nitrite/nitrate levels.
Conclusion: Measurement of EBC biomarkers is a non-invasive and useful way to evaluate airway inflammation in patients with asthma.