The Authors: Thomas Abraham, PhD, is a microscopy specialist and his current research interest centres on structural biology and the developments of novel biomedical research methods based on modern optical principles as applied to heart and lung research; Samuel Wadsworth, PhD, is a postdoctoral fellow who researches the roles of proteases in inflammatory disease and he is also interested in the development of new airway models to test our biological hypotheses; Jon M. Carthy, BSc, is a doctoral candidate and his research interests include wound repair and the extracellular matrix; Dmitri V. Pechkovsky, MD, PhD, holds a research associate position and his research interests are in the areas of interstitial lung diseases and pulmonary fibrosis; Bruce McManus, MD, PhD, is a lead investigator whose investigative program is focused on mechanisms, consequences, detection and prevention of injury and remodelling involved in inflammatory diseases of the heart and blood vessels.
Minimally invasive imaging method based on second harmonic generation and multiphoton excitation fluorescence in translational respiratory research
Article first published online: 23 DEC 2010
© 2010 The Authors. Respirology © 2010 Asian Pacific Society of Respirology
Volume 16, Issue 1, pages 22–33, January 2011
How to Cite
ABRAHAM, T., WADSWORTH, S., CARTHY, J. M., PECHKOVSKY, D. V. and McMANUS, B. (2011), Minimally invasive imaging method based on second harmonic generation and multiphoton excitation fluorescence in translational respiratory research. Respirology, 16: 22–33. doi: 10.1111/j.1440-1843.2010.01898.x
SERIES EDITOR: DARRYL KNIGHT
- Issue published online: 23 DEC 2010
- Article first published online: 23 DEC 2010
- Accepted manuscript online: 5 NOV 2010 03:31AM EST
- Received 1 July 2010; invited to revise 27 September 2010; revised 18 October 2010; accepted 28 October 2010.
- chronic obstructive pulmonary disease;
- extracellular matrix remodelling;
- idiopathic pulmonary fibrosis;
- Lung cancer;
- multi photon excitation fluorescence;
- second harmonic generation;
- tissue engineering
For translational respiratory research including in the development of clinical diagnostic tools, a minimally invasive imaging method, which can provide both cellular and extracellular structural details with sufficient specificity, sensitivity and spatial resolution, is particularly useful. Multiphoton microscopy causes excitation of endogenously fluorescent macromolecular systems and induces highly specific second harmonic generation signals from non-centrosymmetric macromolecules such as fibrillar collagens. Both these signals can be captured simultaneously to provide spatially resolved 3D structural organization of extracellular matrix as well as the cellular morphologies in their native states. Besides briefly discussing the fundamentals of multiphoton excitation fluorescence and harmonic generation signals and the instrumentation details, this review focuses on the specific applications of these imaging modalities in lung structural imaging, particularly morphological features of alveolar structures, visualizing and quantifying extracellular matrix remodelling accompanying emphysematous destructions as well as the IPF, detecting lung cancers and the potential use in the tissue engineering applications.