A NOD2 gene polymorphism is associated with the prevalence and severity of chronic obstructive pulmonary disease in a Japanese population
Version of Record online: 21 DEC 2011
© 2011 The Authors. Respirology © 2011 Asian Pacific Society of Respirology
Volume 17, Issue 1, pages 164–171, January 2012
How to Cite
KINOSE, D., OGAWA, E., HIROTA, T., ITO, I., KUDO, M., HARUNA, A., MARUMO, S., HOSHINO, Y., MURO, S., HIRAI, T., SAKAI, H., DATE, H., TAMARI, M. and MISHIMA, M. (2012), A NOD2 gene polymorphism is associated with the prevalence and severity of chronic obstructive pulmonary disease in a Japanese population. Respirology, 17: 164–171. doi: 10.1111/j.1440-1843.2011.02069.x
- Issue online: 21 DEC 2011
- Version of Record online: 21 DEC 2011
- Accepted manuscript online: 22 SEP 2011 08:46AM EST
- Received 8 May 2011; invited to revise 13 July 2011; revised 2 August 2011; accepted 7 August 2011 (Associate Editor: Robert Young).
- chronic obstructive pulmonary disease;
- nucleotide-binding and oligomerization domain 2;
- single nucleotide polymorphism
Background and objective: Genetic background is thought to be one of the risk factors for development of COPD. Recently, it has been proposed that the innate immune system is involved in the pathophysiology of COPD. We hypothesized that polymorphisms in the nucleotide-binding and oligomerization domain (NOD)1 and NOD2 genes would be associated with the pathogenesis of COPD. In addition, the associations between these single nucleotide polymorphisms (SNPs) and phenotypes of COPD were analysed.
Methods: Japanese COPD patients (n = 228) and non-COPD smokers (n = 101) were recruited from the outpatient clinic at Kyoto University Hospital, Kyoto, Japan. At entry into the study, a blood sample was taken and a pulmonary function test was performed. Genotyping was performed for 6 selected tag SNPs of NOD1 and 5 tag SNPs of NOD2. Further investigations were performed for SNP that were associated with COPD, including baseline gene expression, the relative proportions of splicing variants in whole blood, responses to ligand and enhancement of gene expression in peripheral blood neutrophils stimulated with pro-inflammatory cytokines.
Results: The distribution of NOD2 rs1077861 genotypes differed between Japanese COPD patients and non-COPD smokers (P = 0.036). This SNP was also associated with a lower FEV1 % predicted (57.2 ± 1.8 for TT vs 50.8 ± 2.3 for TA/AA, P = 0.03) and DLCO/VA (2.89 ± 0.1 in TT vs 2.53 ± 0.14 in TA/AA, P = 0.036) in COPD patients. NOD2 gene expression after stimulation with 10 ng/mL of tumour necrosis factor-α for 4 h, was increased to a greater extent in TA/AA genotype than in TT genotype peripheral blood neutrophils (P = 0.015).
Conclusions: The NOD2 rs1077861 SNP may influence the development and progression of COPD in Japanese subjects.