Resistin-like molecule-β is induced following bronchoconstriction of asthmatic airways
Article first published online: 21 SEP 2012
© 2012 The Authors. Respirology © 2012 Asian Pacific Society of Respirology
Volume 17, Issue 7, pages 1094–1100, October 2012
How to Cite
GRAINGE, C., DULAY, V., WARD, J., SAMMUT, D., DAVIES, E., GREEN, B., LAU, L., COTTEY, L., HAITCHI, H.-M., DAVIES, D. E. and HOWARTH, P. H. (2012), Resistin-like molecule-β is induced following bronchoconstriction of asthmatic airways. Respirology, 17: 1094–1100. doi: 10.1111/j.1440-1843.2012.02215.x
- Issue published online: 21 SEP 2012
- Article first published online: 21 SEP 2012
- Accepted manuscript online: 3 JUL 2012 11:30AM EST
- Received 27 October 2011; invited to revise 30 November 2011, 9 January 2012; revised 31 December 2011, 5 March 2012; accepted 29 March 2012 (Associate Editor: Darryl Knight).
- resistin-like molecule-β
Background and objective: Resistin-like molecule-β (RELM-β) is a necessary and sufficient stimulus for airway remodelling in animal models of asthma, but until recently, its role in human disease had not been investigated. The hypothesis that RELM-β expression would increase with increasing asthma severity and further increase following acute bronchoconstrictor challenges has been examined.
Methods: Bronchial biopsies from healthy subjects and patients with mild and severe asthma were immunostained for RELM-β, as were airway biopsies obtained in mild asthmatics before and 4 days after repeated inhalation challenges with either allergen, methacholine or methacholine preceded by salbutamol as a control. Bronchial brushings were also evaluated for RELM-β mRNA.
Results: RELM-β immunoreactivity, which co-localized to airway epithelial cells, increased with disease severity; healthy volunteers, median per cent epithelial area 1.98%, mild asthma 3.49% and severe asthma 5.89% (P < 0.001 between groups). RELM-β immunoreactivity significantly and inversely correlated in asthma with forced expiratory volume in 1 s % predicted (P = 0.005). Acute changes in immunoexpression were evident after repeated inhalation challenge with allergen (2.15 % to 4.35 % (P = 0.01)) and methacholine (4.21 % to 6.16 % (P = 0.01)) but did not change in the salbutamol/methacholine challenge group. These changes correlated with change in basement membrane thickness (r = 0.38, P = 0.02). Epithelial RELM-β gene expression was not altered in asthma.
Conclusions: RELM-β may play an important role not only in animal models of airway remodelling, but also in human airway pathology.