Resistin-like molecule-β is induced following bronchoconstriction of asthmatic airways
Version of Record online: 21 SEP 2012
© 2012 The Authors. Respirology © 2012 Asian Pacific Society of Respirology
Volume 17, Issue 7, pages 1094–1100, October 2012
How to Cite
GRAINGE, C., DULAY, V., WARD, J., SAMMUT, D., DAVIES, E., GREEN, B., LAU, L., COTTEY, L., HAITCHI, H.-M., DAVIES, D. E. and HOWARTH, P. H. (2012), Resistin-like molecule-β is induced following bronchoconstriction of asthmatic airways. Respirology, 17: 1094–1100. doi: 10.1111/j.1440-1843.2012.02215.x
- Issue online: 21 SEP 2012
- Version of Record online: 21 SEP 2012
- Accepted manuscript online: 3 JUL 2012 11:30AM EST
- Received 27 October 2011; invited to revise 30 November 2011, 9 January 2012; revised 31 December 2011, 5 March 2012; accepted 29 March 2012 (Associate Editor: Darryl Knight).
Figure S1 Dot blot performed to check specificity of RELMb antibody. Resistin and RELMb recombinant proteins were loaded onto a hydrated polyvinylidene fluoride membrane in log dilutions and allowed to dry before blocking in 5% milk (Tris buffered saline-Tween). Protein was detected with the same biotinylated antibody to RELMb used for immunohistochemistry at a dilution of 1:1000. Detection was performed using streptavidin horseradish peroxidase at 1:2000 (Sigma, Poole, UK) and visualized using ECL-plus solution (Amersham, Chalfont St Giles, UK) according to manufacturers' instructions.
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