Figure S1 Dot blot performed to check specificity of RELMb antibody. Resistin and RELMb recombinant proteins were loaded onto a hydrated polyvinylidene fluoride membrane in log dilutions and allowed to dry before blocking in 5% milk (Tris buffered saline-Tween). Protein was detected with the same biotinylated antibody to RELMb used for immunohistochemistry at a dilution of 1:1000. Detection was performed using streptavidin horseradish peroxidase at 1:2000 (Sigma, Poole, UK) and visualized using ECL-plus solution (Amersham, Chalfont St Giles, UK) according to manufacturers' instructions.

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