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Relationship of serum interleukin-7 concentration and the coagulation state in children with nephrotic syndrome

Authors


Anna Wasilewska, I Department of Pediatrics, Medical University in Bialystok, 17 Waszyngtona Street, 15-274 Bialystok, Poland. Email: annwasil@interia.pl

Abstract 

Background: Enhanced platelet reactivity may play a significant role in the hypercoagulable state of nephrotic syndrome (NS). Thrombocytosis with platelet aggregation cause the release of some cytokines, among them interleukin-7 (IL-7). The aim of the study was to evaluate serum IL-7 levels in children with the symptoms of NS and to determine a correlation between its concentration and platelet count, other hemostatic factors, and NS intensity.

Methods: The study was performed in two groups. I – the examined group of 26 children with NS (12 boys, 14 girls) aged 6.8 ± 2.1 years, subjected to two examinations: A – before treatment, B – during treatment with prednisone (60 mg/kg 24 h after albuminuria regression); and II – the control group (C) of 20 healthy children. Serum IL-7 level was assayed by enzyme-linked immunosorbent assay method using a R & D Quantikine set.

Results: In group I, IL-7 level in examination A (33.33 ± 33.24 pg/mL) was higher than in the control subjects (P < 0.01). In examination B, IL-7 concentration was reduced to the level of 10.86 ± 5.22 pg/mL and did not differ from the controls (P > 0.05). A positive correlation was observed between IL-7 and platelet count and serum fibrinogen level. A negative correlation was noted with antithrombin III concentration. No correlation was found with serum levels of albumin and cholesterol or urine protein.

Conclusion: In children with NS, serum IL-7 level increases proportionally to the elevated platelet count and other hemostatic components, but shows no correlation with serum albumin or urine protein.

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