Prostaglandin E2 release from isolated bladder strips in rats with spinal cord injury
Version of Record online: 4 APR 2006
International Journal of Urology
Volume 13, Issue 3, pages 271–276, March 2006
How to Cite
MASUNAGA, K., YOSHIDA, M., INADOME, A., IWASHITA, H., MIYAMAE, K. and UEDA, S. (2006), Prostaglandin E2 release from isolated bladder strips in rats with spinal cord injury. International Journal of Urology, 13: 271–276. doi: 10.1111/j.1442-2042.2006.01274.x
- Issue online: 4 APR 2006
- Version of Record online: 4 APR 2006
- Received 18 April 2005; accepted 18 August 2005.
- detrusor overactivity;
- prostaglandin E2;
- spinal cord injury;
Aim: Recent studies have shown that various factors contribute to the increased excitability into the bladder afferent nerves in spinal cord injury (SCI) rats. It has been reported that prostaglandins (PG) act as local modulators of reflex micturition in pathological conditions. In the present study, we measured the amount of PGE2 release from the bladder of chronic SCI rats.
Methods: Spinal cord was transected at the level of T8–9 in adult female Sprague-Dawley rats. After 10 weeks, specimens of the urinary bladder were obtained from SCI rats and sham-injured control rats, and bladder strips were dissected from the bladder. Using an muscle-bath technique and a microdialysis procedure, the dialysate, containing substance released from bladder strips, was collected. Then the amount of PGE2 in the dialysate was measured by radioimmunoassay.
Results: Excretion of urinary PGE2 was significantly higher in SCI rats than in control rats. PGE2 release from bladder strips was significantly higher in SCI rats than in control rats. Removal of urothelium caused significant decreases in PGE2 release in both control and SCI rats. Stretches of the bladder strips caused significant resting tension-dependent increases in PGE2 release from the strips with urothelium.
Conclusions: The present data suggest that bladder urothelium partly contributes to the increase in PGE2 release from the bladder, and that bladder distension may cause increases in PGE2 release in SCI rats.