Original Article: Laboratory Investigation
Partial outlet obstruction in rabbits: Duration versus severity
Article first published online: 10 OCT 2012
© 2012 The Japanese Urological Association
International Journal of Urology
Special Issue: Lower urinary tract dysfunction: From basic science to clinical management
Volume 20, Issue 1, pages 107–114, January 2013
How to Cite
Levin, R. M., Schuler, C., Leggett, R. E., Callaghan, C. and Maknuru, S. (2013), Partial outlet obstruction in rabbits: Duration versus severity. International Journal of Urology, 20: 107–114. doi: 10.1111/j.1442-2042.2012.03184.x
- Issue published online: 2 JAN 2013
- Article first published online: 10 OCT 2012
- Manuscript Accepted: 5 SEP 2012
- Manuscript Received: 9 JUL 2012
- Office of Research and Development Department of the Veterans Affairs
- Capital Region Medical Research Foundation
- free radicals;
- oxidative stress;
Oxidative stress is a major etiology of obstructed bladder dysfunction. The major goal of the current study was to correlate the level of oxidative stress with both the severity and duration of obstruction.
A total of 32 New Zealand White rabbits were divided into four equal groups. Groups 1–3 received partial bladder outlet obstructions by standard methods and survived for 4, 8 or 12 weeks. Group 4 received sham surgery at the end of each time period, isolated strips were taken for contractility studies and the balance of the bladder was frozen as muscle and mucosa for quantification of nitrotyrosine and carbonyl-oxidized proteins derivatized into dinitrophenyl. For each duration, the eight rabbits were divided into three severity groups: mild, intermediate or severe decompensation.
Contractile responses decreased in proportion to both severity and duration. The level of both oxidative products correlated to a much higher degree with the level of severity than the duration. There were significant decreases in the contractile responses in the mild decompensation group, whereas the level of derivatized into dinitrophenyl and nitrotyrosine of the muscle remained at control levels. This was not the case for the 4 weeks obstructed group.
These findings suggest that the etiology for the mechanism of contractile dysfunction is not an oxidative stress.