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Effect of human vitreous and hyalocyte-derived factors on vascular endothelial cell growth
Article first published online: 7 NOV 2007
DOI: 10.1111/j.1442-9071.1997.tb01758.x
Issue
1442-9071/asset/cover.gif?v=1&s=7c6769e05691a4581ae173da02433f6f6fe197f7 "Australian and New Zealand Journal of Ophthalmology")
Australian and New Zealand Journal of Ophthalmology
Volume 25, Issue 4, pages 57–60, November 1997
Additional Information
How to Cite
Zhu, M., Penfold, P., Madigan, M. and Billson, F. (1997), Effect of human vitreous and hyalocyte-derived factors on vascular endothelial cell growth. Australian and New Zealand Journal of Ophthalmology, 25: 57–60. doi: 10.1111/j.1442-9071.1997.tb01758.x
Publication History
- Issue published online: 7 DEC 2009
- Article first published online: 7 NOV 2007
- Abstract
- References
- Cited By
Keywords:
- cell growth;
- human umbilical vein endothelial cells;
- hyalocytes;
- vitreous
ABSTRACT
Purpose:
In the present study we have examined the effects of human vitreous and hyalocyte-conditioned medium on human umbilical vein endothelial cells (HUVEC).
Methods:
Human umbilical vein endothelial cells were cultured and treated in quadruplicate with human vitreous and hyalocyte-conditioned medium at different concentrations. Cell numbers were counted on days 1, 3, 5 and 7. Morphological changes and the viability of cells after treatment were also monitored.
Results:
The results indicate that both human vitreous and hyalocyte-conditioned medium inhibit proliferation and reduce the viability of HUVEC in vitro. These inhibitory effects were dose- and time-dependent.
Conclusions:
The observations suggest that human hyalo-cytes and vitreous contain anti-angiogenic factors that influence vascular endothelial cell growth. These results, combined with those of previous studies, may yield important information about the functional role of vitreous and hyalocytes in intraocular vascular regression.