Effect of stem cells and fibrin concentration on the vascularization of the Medpor orbital implant
Version of Record online: 21 JUN 2010
© 2010 The Authors. Clinical and Experimental Ophthalmology © 2010 Royal Australian and New Zealand College of Ophthalmologists
Clinical & Experimental Ophthalmology
Volume 38, Issue 9, pages 885–891, December 2010
How to Cite
Lee, S.-G., Yang, J.-W., Park, S.-G. and Yang, Y.-I. (2010), Effect of stem cells and fibrin concentration on the vascularization of the Medpor orbital implant. Clinical & Experimental Ophthalmology, 38: 885–891. doi: 10.1111/j.1442-9071.2010.02355.x
- Issue online: 8 DEC 2010
- Version of Record online: 21 JUN 2010
- Received 17 February 2010; accepted 1 June 2010.
- adipose-derived adult stem cell (ADASC);
- porous polyethylene orbital implant (Medpor)
Background: To determine the effect of adipose-derived adult stem cells (ADASCs) and optimal concentration of fibrin on fibrovascular ingrowth into porous polyethylene orbital implants (Medpor).
Methods: Medpor sheet treated with O.25% fibrin only and ADASCs in mixtures containing fibrin (0.25%, 0.5% or 1.25%) were applied to a Medpor sheet and implanted in the back of each of 20 athymic nude mice. After 10 days, implants were removed and observed for fibrovascularization and stability. Haemoglobin, collagen and cellular DNA content were determined in quantitative assays.
Results: Haemoglobin, collagen and cellular DNA levels were significantly higher in ADASC group than in the cell-free implant (0.25% fibrin only) group (P < 0.01). The level of haemoglobin and collagen content was significantly higher in the ADASC + 0.5% fibrin group among the ADASC and fibrin mixtures (P < 0.01).
Conclusion: ADASCs significantly improved fibrovascularization on Medpor compared with implants alone. Fibrin, used together with ADASCs to potentiate fibrovascularization, was most effective at concentrations of 0.5%.