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Differential gene expression pattern of diabetic rat retinas after intravitreal injection of erythropoietin

Authors

  • Qing Chu MMS,

    1. Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
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  • Jingfa Zhang PhD,

    1. Tongji Eye Institute and Department of Regenerative Medicine, Tongji University School of Medicine
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  • Yalan Wu MS,

    1. Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
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  • Yu Zhang MD,

    1. Department of Ophthalmology, Huashan Hospital Affiliated to Fudan University, Shanghai
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  • Guoxu Xu MMS,

    1. Department of Ophthalmology, Second Affiliated Hospital of Soochow University, Suzhou, China
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    • Guoxu Xu, Weiye Li and Guo-Tong Xu contributed equally to this study.

  • Weiye Li MD PhD,

    1. Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
    2. Tongji Eye Institute and Department of Regenerative Medicine, Tongji University School of Medicine
    3. Department of Ophthalmology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA
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    • Guoxu Xu, Weiye Li and Guo-Tong Xu contributed equally to this study.

  • Guo-Tong Xu MD PhD

    Corresponding author
    1. Laboratory of Clinical Visual Sciences, Institute of Health Sciences, Shanghai JiaoTong University School of Medicine and Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
    2. Tongji Eye Institute and Department of Regenerative Medicine, Tongji University School of Medicine
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    • Guoxu Xu, Weiye Li and Guo-Tong Xu contributed equally to this study.


Dr Guo-Tong Xu, Tongji Eye Institute and Department of Regenerative Medicine, Tongji University School of Medicine, 1239 Siping Road, Medical School Building, Room 521, Shanghai 200092, China. Email: gtxu@tongji.edu.cn

Abstract

Background:  To profile the pattern of gene expression in diabetic rat retinas with or without intravitreal injection of erythropoietin.

Design:  By using streptozotocin-induced diabetic rats, after intravitreal injection of erythropoietin, neurosensory retinas were collected to determine the effect of erythropoietin on gene expression.

Participants:  Three groups of Sprague–Dawley rats were studied: normal control (15), diabetic rats with saline injection (15) and diabetic rats with intravitreal erythropoietin treatment (15).

Methods:  Diabetes was induced by intra-peritoneal injection of streptozotocin. Intravitreal injection of erythropoietin was performed at the following time points: 0, 30 and 120 days after diabetes onset. Four days after each injection at above-mentioned time points, the retinas were harvested for microarray assay. The real-time PCR was used to evaluate the microarray data.

Results:  Genes encoding inflammatory factors, such as interleukin-2 and interleukin-11, which were upregulated in the diabetic retinas, were restored after erythropoietin treatment. Genes encoding pro-apoptotic effectors, like Tnfrsf5, Bid3 and Bcl2l1, were also upregulated in diabetic rats and attenuated in erythropoietin-treated group. In addition, real-time PCR were employed to confirm the changes of the genes Trex2, G1P2, DHX58, RGD1311906 and LOC689064, which have not been reported in diabetic retinopathy.

Conclusions:  Intravitreal erythropoietin treatment is able to normalize the gene expression responsible for pro-apoptotic and inflammatory responses noted in diabetic retinas.

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