Geldanamycin is effective in the treatment of herpes simplex virus epithelial keratitis in a rabbit model
Article first published online: 23 MAY 2011
© 2011 The Authors. Clinical and Experimental Ophthalmology © 2011 Royal Australian and New Zealand College of Ophthalmologists
Clinical & Experimental Ophthalmology
Volume 39, Issue 8, pages 779–783, November 2011
How to Cite
Wu, X., Tao, P. and Nie, H. (2011), Geldanamycin is effective in the treatment of herpes simplex virus epithelial keratitis in a rabbit model. Clinical & Experimental Ophthalmology, 39: 779–783. doi: 10.1111/j.1442-9071.2011.02558.x
- Issue published online: 3 NOV 2011
- Article first published online: 23 MAY 2011
- Accepted manuscript online: 15 MAR 2011 05:16AM EST
- Received 5 October 2010; accepted 2 March 2011.
- herpes simplex virus type 1;
- rabbit model
Background: To evaluate the efficacy of geldanamycin eye drops against herpes simplex virus epithelial keratitis in a rabbit model.
Methods: New Zealand white rabbits were randomized into four groups and infected with herpes simplex virus type 1; geldanamycin topical eye drops was initiated 24 h after the infection and maintained for 12 consecutive days. Four groups of rabbits received 5 µg/mL geldanamycin, 10 µg/mL geldanamycin, 0.1% acyclovir and escipient (a kind of artificial tears), respectively. The severity of herpes simplex virus type 1 epithelial keratitis was measured by slit-lamp and scored for statistics analysis. The virus shedding in eye swabs was isolated, and tissue culture infective dose (TCID50) was determined.
Results: Geldanamycin (10 µg/mL) treatment reduced significantly the severity of herpes simplex virus type 1 epithelial keratitis than the other three groups. Geldanamycin (5 µg/mL) was as effective as acyclovir (0.1%) treatment. The effect of geldanamycin against herpes simplex virus type 1 epithelial keratitis correlated with accelerated clearance of virus of the rabbits.
Conclusion: Geldanamycin is a promising treatment option against herpes simplex virus type 1 epithelial keratitis. Geldanamycin (10 µg/mL) is better than acyclovir and geldanamycin (5 µg/mL) in the rabbit model. The optimal concentration of this drug in human is still to be determined.