Competing/conflicts of interest: No stated conflict of interest.
Enhanced age-related cataract in copper-zinc superoxide dismutase null mice
Article first published online: 4 JUN 2012
© 2012 The Authors. Clinical and Experimental Ophthalmology © 2012 Royal Australian and New Zealand College of Ophthalmologists
Clinical & Experimental Ophthalmology
Volume 40, Issue 8, pages 813–820, November 2012
How to Cite
Olofsson, E. M., Marklund, S. L. and Behndig, A. (2012), Enhanced age-related cataract in copper-zinc superoxide dismutase null mice. Clinical & Experimental Ophthalmology, 40: 813–820. doi: 10.1111/j.1442-9071.2012.02794.x
Funding sources: This study was funded by grants from the Swedish Research Council, Synfrämjandets Research Fund, and the KMA Fund.
- Issue published online: 7 NOV 2012
- Article first published online: 4 JUN 2012
- Accepted manuscript online: 19 MAR 2012 09:42AM EST
- Received 2 October 2011; accepted 8 March 2012.
- knockout model;
- reactive oxygen species;
- copper-zinc superoxide dismutase
Background: As the lens is constantly exposed to light and oxygen that generate harmful reactive oxygen species, the importance of the intracellular antioxidant enzyme copper-zinc superoxide dismutase for the protection against age-related cataract development was explored.
Methods: The development of lens opacities and the lens oxidative status were studied in different age groups of mice lacking copper-zinc superoxide dismutase and in wild-type mice. The lens opacities were quantified from lens photographs using digital image analysis. Thereafter, the lenses were homogenized and analysed regarding their contents of reduced glutathione and protein carbonyls suggestive of protein oxidation.
Results: The 18-week-old mice of both genotypes had clear lenses. At 1 year of age, the copper-zinc superoxide dismutase null mice had developed cortical lens opacities, whereas the wild-type mice did not show equivalent changes until 2 years of age. The lens contents of glutathione decreased only in the 2-year-old wild-type mice, whereas the carbonyls increased over time without any differences between the two genotypes.
Conclusions: This study indicates that the lack of copper-zinc superoxide dismutase may accelerate age-related lens opacity development and that intracellular superoxide-derived oxidative stress may be damaging to the lens during ageing. Participation of the anti-oxidant enzyme copper-zinc superoxide dismutase in the protection against age-related cataract was thus suggested.