Competing/conflicts of interest: No stated conflict of interest.
Characterization of lacrimal sac histology: an immunohistochemical study
Article first published online: 11 JUL 2012
© 2012 The Authors. Clinical and Experimental Ophthalmology © 2012 Royal Australian and New Zealand College of Ophthalmologists
Clinical & Experimental Ophthalmology
Volume 40, Issue 9, pages 869–873, December 2012
How to Cite
Gupta, A., Prabhakaran, V. C., Dodd, T. and Selva, D. (2012), Characterization of lacrimal sac histology: an immunohistochemical study. Clinical & Experimental Ophthalmology, 40: 869–873. doi: 10.1111/j.1442-9071.2012.02818.x
Funding sources: No stated funding sources.
- Issue published online: 6 DEC 2012
- Article first published online: 11 JUL 2012
- Accepted manuscript online: 18 MAY 2012 03:52AM EST
- Received 5 January 2012; accepted 18 March 2012.
- lacrimal sac
Background: A prospective observational study in a university hospital setting to study the immunohistochemical (IHC) characteristics of non-neoplastic human lacrimal sac epithelium.
Methods: Twenty paraffin-embedded specimens of human lacrimal sac were studied using monospecific monoclonal antibodies to 34 beta E12, cell adhesion molecule (CAM 5.2), epithelial membrane antigen (EMA), cytokeratins (CK) 7 and 20, estrogen receptor and progesterone receptor. The distribution and histologic location of IHC staining were examined qualitatively, and the IHC stains scored as positive (+) or negative (−).
Results: The haematoxylin–eosin stains were reviewed for tissue morphology. All 20 specimens were positive for 34 beta E12, CAM 5.2, EMA and CK 7 and negative for CK 20, estrogen receptor and progesterone receptor.
Conclusion: To our knowledge, this is the first study to characterize the IHC properties of human lacrimal sac epithelium. This epithelium appears to possess consistent IHC properties as it stains for 34 beta E12, CAM 5.2, EMA and CK 7 and this information would be potentially useful in differentiating tumours arising in the region of the lacrimal sac.