Competing/conflicts of interest: No stated conflict of interest.
Preventive effect of danshensu on selenite-induced cataractogenesis in cultured rat lens
Article first published online: 4 SEP 2012
© 2012 The Authors. Clinical and Experimental Ophthalmology © 2012 Royal Australian and New Zealand College of Ophthalmologists
Clinical & Experimental Ophthalmology
Volume 41, Issue 2, pages 172–179, March 2013
How to Cite
Qi, H.-P., Wei, S.-Q., Zhang, L.-Q., Gao, X.-C., Yu, N.-N., Bi, S. and Cui, H. (2013), Preventive effect of danshensu on selenite-induced cataractogenesis in cultured rat lens. Clinical & Experimental Ophthalmology, 41: 172–179. doi: 10.1111/j.1442-9071.2012.02837.x
Funding sources: No stated funding sources.
- Issue published online: 18 MAR 2013
- Article first published online: 4 SEP 2012
- Accepted manuscript online: 19 JUN 2012 10:42AM EST
- Received 12 January 2012; accepted 10 May 2012.
- cultured rat lens;
- selenite-induced cataract
Background: To investigate the preventive effect of danshensu on the selenite-induced opacification of cultured rat lenses.
Methods: Isolated lens were divided into three groups with eight lenses in each group. Group I: lenses were incubated with M199 medium alone; Group II: incubated in M199 containing 200 µmol/L sodium selenite; Group III: incubated in M199 containing 200 µmol/L sodium selenite and 500 µmol/L danshensu. Selenite was administered on the third day, and danshensu treatment was from the second to the fifth day. Cataracts development was observed using an inverted microscope, and the lenses were analysed for total anti-oxidative capabilities, mean activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase; levels of reduced glutathione; malondialdehyde; and total sulfhydryl content.
Results: All lenses in Group I were clear, whereas all lenses in Group II developed dense vacuolization and opacification. In Group III, 25% lenses revealed minimal vacuolization, and 75% showed no opacification or vacuolization. Total anti-oxidative capabilities and the mean activities of anti-oxidant enzymes superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione S-transferase; levels of glutathione; and total sulfhydryl content were elevated, and the level of malondialdehyde was decreased following treatment with danshensu compared with Group II.
Conlusion: The anti-oxidative properties of danshensu may play a major role in its contribution to the anticataract effect.