Abstract This study describes the floristics and structure of a 0.95-ha lowland tropical rainforest plot at the Australian Canopy Crane Research Facility at Cape Tribulation, Queensland. Five years of post-cyclonic change in forest floristics and structure following the passage of Tropical Cyclone ‘Rona’ in February 1999 are examined. Local and regional variation in tropical rainforest is examined in comparison with other lowland plots established nearby and mid-elevation plots located elsewhere in north Queensland at Eungella, Paluma and the Atherton Tablelands. These plots are placed in a broader Australasian context along with lowland rainforest plots at Baitabag and Oomsis, Papua New Guinea. The 2005 survey found 680 stems of 82 species ≥10 cm d.b.h. on the crane plot, an increase of 30.3% in stems and 16.4% of species in the 5 years since the previous survey. The most abundant families were Meliaceae, Euphorbiaceae, Lauraceae, Myrtaceae and Apocynaceae and the most abundant species were Cleistanthus myrianthus, Alstonia scholaris, Myristica insipida, Normanbya normanbyi and Rockinghamia angustifolia. Temporal floristic and structural variation suggests that the crane site remains in an active stage of post-cyclonic recovery. Local spatial variability in floristics and structure at Cape Tribulation exceeded the variation exhibited by a single plot over a period of 5 years, despite the impact of Cyclone Rona. This finding suggests a high degree of temporal stability within this stand of rainforest despite frequent catastrophic disturbances. The rainforests of Cape Tribulation constitute a relatively unique floristic community when observed in an Australasian context. Variation in rainforest community composition across the region shows the importance of biogeographical connections, the impacts of local topography, environmental conditions and disturbance history.