Induction of centrum defects in amberjack Seriola dumerili by exposure of embryos to hypoxia

  1. Yoshifumi SAWADA1,*,
  2. Manabu HATTORI1,
  3. Masahide ITEYA2,
  4. Yasuaki TAKAGI3,
  5. Kazuhiro URA3,
  6. Manabu SEOKA4,
  7. Keitaro KATO5,
  8. Michio KURATA6,
  9. Hirofumi MITATAKE6,
  10. Shigekazu KATAYAMA6,
  11. Hidemi KUMAI4

Article first published online: 11 APR 2006

DOI: 10.1111/j.1444-2906.2006.01158.x

Issue

Fisheries Science

Fisheries Science

Volume 72, Issue 2, pages 364–372, April 2006

Additional Information

How to Cite

SAWADA, Y., HATTORI, M., ITEYA, M., TAKAGI, Y., URA, K., SEOKA, M., KATO, K., KURATA, M., MITATAKE, H., KATAYAMA, S. and KUMAI, H. (2006), Induction of centrum defects in amberjack Seriola dumerili by exposure of embryos to hypoxia. Fisheries Science, 72: 364–372. doi: 10.1111/j.1444-2906.2006.01158.x

Author Information

  1. 1

    Ohshima Experiment Station, Fisheries Laboratory, Kinki University, Kushimoto, Wakayama 649-3633,

  2. 2

    Faculty of Agriculture, Kinki University, Nakamachi, Nara,

  3. 3

    Graduate School of Fisheries Science, Hokkaido University, Hakodate, Hokkaido,

  4. 4

    Uragami Experiment Station, Fisheries Laboratory, Kinki University, Nachikatsuura, Wakayama 649-5145,

  5. 5

    Shirahama Experiment Station, Fisheries Laboratory, Kinki University, Shirahama, Wakayama 649-2211, and

  6. 6

    Fish Nursery Center, Kinki University, Kushimoto, Wakayama 649-3633, Japan

* *Tel: 81-735-65-0501. Fax: 81-735-65-0974. Email: YoshifumiSawada@za.ztv.ne.jp

Publication History

  1. Issue published online: 11 APR 2006
  2. Article first published online: 11 APR 2006
  3. Received 22 July 2005. Accepted 31 October 2005.

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Keywords:

  • amberjack;
  • centrum defects;
  • hypoxia;
  • Seriola dumerili;
  • somitic disturbances;
  • somitogenesis

ABSTRACT:  Artificially hatched Seriola species have the problem of malformation, mainly in their vertebrae, head, and mouth parts. To clarify the cause of vertebral malformation, the effects of hypoxia during embryogenesis on the induction of centrum defects was investigated in artificially hatched amberjack Seriola dumerili. Firstly, 7-somite stage embryos were exposed to waters of 0, 12.5, 25, 50, and 100% dissolved oxygen (DO) for 0.5, 1, 2, 3, 4, and 5 h to confirm the effective dose (DO concentration and duration of exposure) of hypoxia that induces somitic disturbances in newly hatched larvae. Exposure of embryos to 12.5% DO concentration for longer than 0.5 h induced somitic disturbances. Following this result, centrum defects in juveniles were investigated by an induction experiment with embryos exposed to 12.5% DO for 2 h at the gastrula, 1- or 2-somite, 10-somite, 15-somite, or heart beating stage. This experiment revealed that centrum defects were induced only during somitogenesis, and somitic disturbances were the premonitory symptom of centrum defects. These results indicate hypoxia during somitogenesis as a possible cause of centrum defects in amberjack.

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