Present address: Department of Biological Science, Faculty of Science, Nara Women's University, Kitauoya-nishimachi, Nara 630-8506, Japan.
Low genetic variability in an endangered population of fiddler crab Uca arcuata on Okinawajima Island: analysis of mitochondrial DNA
Article first published online: 31 MAR 2008
© 2008 Japanese Society of Fisheries Science
Volume 74, Issue 2, pages 330–340, April 2008
How to Cite
AOKI, M., NARUSE, T., CHENG, J.-H., SUZUKI, Y. and IMAI, H. (2008), Low genetic variability in an endangered population of fiddler crab Uca arcuata on Okinawajima Island: analysis of mitochondrial DNA. Fisheries Science, 74: 330–340. doi: 10.1111/j.1444-2906.2008.01529.x
- Issue published online: 31 MAR 2008
- Article first published online: 31 MAR 2008
- Received 21 May 2007. Accepted 16 November 2007.
- fiddler crab;
- genetic variability;
- mitochondrial DNA;
- population structure;
- Uca arcuata
ABSTRACT: Restriction fragment length polymorphism analysis was performed on polymerase chain reaction-amplified DNA fragments containing the D-loop, ND2, and CO I genes of fiddler crab Uca arcuata mitochondrial DNA. In total, 316 individuals from six populations in Japan and two populations in Taiwan were analyzed using five restriction endonucleases (AfaI, BcnI, Cfr13I, HaeIII and HinfI), yielding 85 haplotypes. Samples were taken from Nakagusuku Bay, Okinawajima Island, which is the only known distribution of U. arcuata in the Ryukyu Archipelago. The Okinawajima Island population is isolated geographically from others and showed a marked low genetic variability (h = 0.2539, π = 0.0005) and significant differentiation from other population samples in haplotype composition. We suggest that a substantial decrease in the genetic variability of the Okinawajima Island population was caused by genetic drift under the conditions of small population size and low gene flow from other populations. It is important to conserve the intertidal zone in Nakagusuku Bay for the maintenance of this endangered population.