Availability of genetically modified feed ingredient: investigations of ingested foreign DNA in rainbow trout Oncorhynchus mykiss
Article first published online: 31 MAR 2008
© 2008 Japanese Society of Fisheries Science
Volume 74, Issue 2, pages 380–390, April 2008
How to Cite
CHAINARK, P., SATOH, S., HIRONO, I., AOKI, T. and ENDO, M. (2008), Availability of genetically modified feed ingredient: investigations of ingested foreign DNA in rainbow trout Oncorhynchus mykiss. Fisheries Science, 74: 380–390. doi: 10.1111/j.1444-2906.2008.01535.x
- Issue published online: 31 MAR 2008
- Article first published online: 31 MAR 2008
- Received 10 August 2007. Accepted 2 November 2007.
- CaMV 35S promoter;
- gastrointestinal tract;
- genetically modified defatted soybean meal;
- in situ hybridization
ABSTRACT: Foreign DNA fragments from genetically modified defatted soybean meal (GM SBM) in rainbow trout was traced by nested polymerase chain reaction (PCR) and located by in situ hybridization. Either a GM or non-GM SBM formulated diet (42% protein) was fed to fish (average weight 50.5 g) for 2 weeks. The degradation results showed that the cauliflower mosaic virus 35S promoter (220 bp) fragment was detected in the contents of digestive system only in fish fed the GM SBM diet, and it was not detected on the third day after changing the diet to the non-GM SBM diet. For the possible transferal results, the promoter fragment was detected in the leukocyte, head kidney and muscle only of fish fed the GM SBM diet; it was not detected on the fifth day after changing the diet to the non-GM SBM diet. These results suggest that a foreign DNA fragment was not completely degraded and might be taken up into organs through the gastrointestinal tract. However, foreign DNA was not detected after the withdrawal period. Thus, the data show that uptake of DNA from GM SBM might not remain in the tissues of fish fed GM SBM diet.