Evaluation of fertilizing capacity of cryopreserved rainbow trout sperm
Article first published online: 2 JUN 2008
© 2008 Japanese Society of Fisheries Science
Volume 74, Issue 3, pages 621–626, June 2008
How to Cite
KUROKURA, H. and MOO, K. (2008), Evaluation of fertilizing capacity of cryopreserved rainbow trout sperm. Fisheries Science, 74: 621–626. doi: 10.1111/j.1444-2906.2008.01566.x
- Issue published online: 2 JUN 2008
- Article first published online: 2 JUN 2008
- Received 11 May 2007. Accepted 3 December 2007.
- fertilizing capacity;
- rainbow trout;
- sperm motility
ABSTRACT: Experimental insemination was performed using artificially produced low-motility sperm. A mathematical model was applied to the results of the insemination in order to clarify the relationship between sperm motility, the density of sperm and the fertilization rate of eggs. In the model, the probability of fertilization by individual spermatozoa was a function of sperm density in the insemination solution. The results showed that the probability of fertilization clearly decreased with increased sperm density, and the maximum possible fertilizing rate by increasing the sperm density was constrained by the proportion of motile sperm (% motility). The model was also applied to the results of insemination tests of cryopreserved sperm in order to evaluate the fertilizing capacity of cryopreserved sperm. It was proven that cryopreserved sperm needed a higher density to obtain the maximum fertilization rate compared with fresh sperm, and it was anticipated that the ratio of the motile inseminated cryopreserved sperm should be more than 5.0% to achieve an egg fertilization rate greater than 90%.