Present address: Research Institute of Marine Bioresources, Fukuyama University, Onomichi, Hiroshima 722-2101, Japan.
Direct evidence of multiple paternities in natural population of viviparous Japanese surfperch by allelic markers of microsatellite DNA loci
Article first published online: 5 OCT 2008
© 2008 Japanese Society of Fisheries Science
Volume 74, Issue 5, pages 976–982, October 2008
How to Cite
TAKAGI, M., SAKAI, K. and TANIGUCHI, N. (2008), Direct evidence of multiple paternities in natural population of viviparous Japanese surfperch by allelic markers of microsatellite DNA loci. Fisheries Science, 74: 976–982. doi: 10.1111/j.1444-2906.2008.01615.x
- Issue published online: 5 OCT 2008
- Article first published online: 5 OCT 2008
- Received 9 October 2007. Accepted 17 March 2008.
- microsatellite DNA;
- multiple paternities;
- viviparous surfperch
This study was performed to obtain information on the occurrence of multiple paternities in three species of viviparous Japanese surfperch using allelic markers of microsatellite DNA loci. Direct evidence for multiple fertilizations was established by reconstructing paternal genotypes from the progeny of gravid females. Multiple paternities were ascertained in five of 10 broods of Ditrema temmincki and in three of nine broods of Neoditrema ransonneti, but not in Ditrema viride. The number of patrilines detected in the progeny of D. temmincki and N. ransonneti females were two or three, respectively, as determined by the GERUD v2.0 algorithm for reconstructing parental genotypes from half-sib progeny arrays.