Present address: Foods Development and Research Section, Prime Delica Co., Ltd, Sagamihara, Kanagawa 228-0828, Japan.
Characterization and expression of Saprospira cytoplasmic fibril protein (SCFP) gene from algicidal Saprospira spp. strains
Article first published online: 5 OCT 2008
© 2008 Japanese Society of Fisheries Science
Volume 74, Issue 5, pages 1109–1117, October 2008
How to Cite
YOSHIKAWA, T., NAKAHARA, M., TABATA, A., KOKUMAI, S., FURUSAWA, G. and SAKATA, T. (2008), Characterization and expression of Saprospira cytoplasmic fibril protein (SCFP) gene from algicidal Saprospira spp. strains. Fisheries Science, 74: 1109–1117. doi: 10.1111/j.1444-2906.2008.01630.x
- Issue published online: 5 OCT 2008
- Article first published online: 5 OCT 2008
- Received 26 October 2007. Accepted 7 March 2008.
- cytoplasmic fibril protein (SCFP);
- gliding motility;
- phage tail sheath protein;
- Saprospira sp
The Saprospira sp. strain SS98-5 cells form colonies on a nutrient-rich agar medium, but are motile by gliding under low-nutrient condition and then numerous microtubule-like fibril structures are found intracellularly. The fibril structures are composed of a proteinous subunit SCFP; this gene was cloned previously. Here, the organization of ORFs adjacent to the SS98-5 SCFP gene and its transcription were investigated, and a SCFP gene homolog was cloned from Saprospira sp. SS03-4, a relative strain of SS98-5. The SCFP gene was also encoded within the SS03-4 chromosome, and grouped into a phage tail sheath protein family, suggesting its bacteriophage genome origin. Four ORFs adjacent to the SCFP gene were identified and their organization was in common with several prokaryotes. The SCFP mRNA was detected by reverse transcriptase polymerase chain reaction from gliding and non-gliding cells, implying that the SCFP gene was transcribed independent of existence or absence of the fibril structures.