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Characterization and expression of Saprospira cytoplasmic fibril protein (SCFP) gene from algicidal Saprospira spp. strains

Authors

  • Takeshi YOSHIKAWA,

    Corresponding author
    1. Department of Biochemistry and Technology of Aquatic Resources, Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, and
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  • Miyuki NAKAHARA,

    1. Department of Biochemistry and Technology of Aquatic Resources, Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, and
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    • a

      Present address: Foods Development and Research Section, Prime Delica Co., Ltd, Sagamihara, Kanagawa 228-0828, Japan.

  • Ayano TABATA,

    1. Department of Biochemistry and Technology of Aquatic Resources, Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, and
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    • b

      Present address: Pharmacokinetics and Bioanalysis Center, Shin Nippon Biomedical Laboratories, Ltd, Kainan, Wakayama 642-0017, Japan.

  • Shingo KOKUMAI,

    1. Department of Biochemistry and Technology of Aquatic Resources, Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, and
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  • Go FURUSAWA,

    1. United Graduate School of Agricultural Science, Kagoshima University, Kagoshima 890-0065, Japan
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    • c

      Present address: Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844, USA.

  • Taizo SAKATA

    1. Department of Biochemistry and Technology of Aquatic Resources, Faculty of Fisheries, Kagoshima University, Kagoshima 890-0056, and
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*Tel: 81-99-286-4191.
Fax: 81-99-286-4191. Email: yoshi@fish.kagoshima-u.ac.jp

ABSTRACT

The Saprospira sp. strain SS98-5 cells form colonies on a nutrient-rich agar medium, but are motile by gliding under low-nutrient condition and then numerous microtubule-like fibril structures are found intracellularly. The fibril structures are composed of a proteinous subunit SCFP; this gene was cloned previously. Here, the organization of ORFs adjacent to the SS98-5 SCFP gene and its transcription were investigated, and a SCFP gene homolog was cloned from Saprospira sp. SS03-4, a relative strain of SS98-5. The SCFP gene was also encoded within the SS03-4 chromosome, and grouped into a phage tail sheath protein family, suggesting its bacteriophage genome origin. Four ORFs adjacent to the SCFP gene were identified and their organization was in common with several prokaryotes. The SCFP mRNA was detected by reverse transcriptase polymerase chain reaction from gliding and non-gliding cells, implying that the SCFP gene was transcribed independent of existence or absence of the fibril structures.

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