Separation and characterization of connective tissue cells expressing 43-kDa zinc-binding protein from common carp
Article first published online: 5 DEC 2008
© 2008 Japanese Society of Fisheries Science
Volume 74, Issue 6, pages 1322–1329, December 2008
How to Cite
CHEN, Y. H., LIAO, H. J. and JENG, S. S. (2008), Separation and characterization of connective tissue cells expressing 43-kDa zinc-binding protein from common carp. Fisheries Science, 74: 1322–1329. doi: 10.1111/j.1444-2906.2008.01658.x
- Issue published online: 5 DEC 2008
- Article first published online: 5 DEC 2008
- Received 14 February 2008. Accepted 8 May 2008.
- common carp;
- connective tissue cells;
- fish cells;
- zinc-binding protein;
To separate the connective tissue cells expressing the 43-kDa zinc-binding protein from the common carp, mucosa of the digestive tract of the fish was removed by scraping with a glass slide, and the de-mucosa tissue was digested with a collagenase type IV solution. The cells collected from the collagenase-treated suspension were rather homogeneous, and more than 90% of the cells were round with a diameter of approximately 6 μm. Significant quantities of the 43 kDa zinc-binding protein were shown to be present on the cell surface of the approximately 6-μm cells. The mean zinc concentration in the cells was found to be 2.21 μg zinc/106 cells, which is approximately 20–30 times higher than that found in the other three fish species (grass carp, silver carp, tilapia) studied. The present work may provide a basis for the culture of these cells.