Aspirin induces apoptosis in oesophageal cancer cells by inhibiting the pathway of NF-kappaB downstream regulation of cyclooxygenase-2

Authors

  • Jun-Feng Liu,

    1. *Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China, †Department of Surgery, Royal Adelaide Hospital, Adelaide University, ‡School of Nursing and Midwifery, Flinders University, Adelaide, Australia, §Department of Endocrinology and ¶Scientific Research Centre, Fourth Hospital, Hebei Medical University, Shijiazhuang, China
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  • Glyn G. Jamieson,

    1. *Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China, †Department of Surgery, Royal Adelaide Hospital, Adelaide University, ‡School of Nursing and Midwifery, Flinders University, Adelaide, Australia, §Department of Endocrinology and ¶Scientific Research Centre, Fourth Hospital, Hebei Medical University, Shijiazhuang, China
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  • Paul A. Drew,

    1. *Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China, †Department of Surgery, Royal Adelaide Hospital, Adelaide University, ‡School of Nursing and Midwifery, Flinders University, Adelaide, Australia, §Department of Endocrinology and ¶Scientific Research Centre, Fourth Hospital, Hebei Medical University, Shijiazhuang, China
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  • Gui-Jun Zhu,

    1. *Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China, †Department of Surgery, Royal Adelaide Hospital, Adelaide University, ‡School of Nursing and Midwifery, Flinders University, Adelaide, Australia, §Department of Endocrinology and ¶Scientific Research Centre, Fourth Hospital, Hebei Medical University, Shijiazhuang, China
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  • Shao-Wei Zhang,

    1. *Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China, †Department of Surgery, Royal Adelaide Hospital, Adelaide University, ‡School of Nursing and Midwifery, Flinders University, Adelaide, Australia, §Department of Endocrinology and ¶Scientific Research Centre, Fourth Hospital, Hebei Medical University, Shijiazhuang, China
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  • Tie-Nian Zhu,

    1. *Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China, †Department of Surgery, Royal Adelaide Hospital, Adelaide University, ‡School of Nursing and Midwifery, Flinders University, Adelaide, Australia, §Department of Endocrinology and ¶Scientific Research Centre, Fourth Hospital, Hebei Medical University, Shijiazhuang, China
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  • Bao-En Shan,

    1. *Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China, †Department of Surgery, Royal Adelaide Hospital, Adelaide University, ‡School of Nursing and Midwifery, Flinders University, Adelaide, Australia, §Department of Endocrinology and ¶Scientific Research Centre, Fourth Hospital, Hebei Medical University, Shijiazhuang, China
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  • Qi-Zhang Wang

    1. *Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China, †Department of Surgery, Royal Adelaide Hospital, Adelaide University, ‡School of Nursing and Midwifery, Flinders University, Adelaide, Australia, §Department of Endocrinology and ¶Scientific Research Centre, Fourth Hospital, Hebei Medical University, Shijiazhuang, China
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  • J.-F. Liu MD; G. G. Jamieson MS, FRACS; P. A. Drew PhD; G.-J. Zhu MD; S.-W. Zhang MMed; T.-N. Zhu MD; B.-E. Shan MD; Q.-Z. Wang BMed.

Professor Jun-Feng Liu, Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, 12 Jiankang Road, Shijiazhuang 050011, China.
Email: liujf@heinfo.net

Abstract

Background:  Aspirin has potential in the prevention or treatment of oesophageal cancer, the seventh most common cancer in the world, but its mechanism of action is still not certain.

Methods:  The oesophageal squamous cell carcinoma cell line TE-13 was cultured with aspirin at different concentrations or for different times. Proliferation and apoptosis were measured by MTT reduction and flow cytometry. Expression of COX-2 mRNA was measured by RT-PCR and COX-2 protein levels with Western blot analysis. Nuclear NF-kappaB and cytoplasmic IkappaB protein levels were determined by electrophoretic mobility shift assay and Western blot, respectively.

Results:  Aspirin significantly inhibited cell proliferation and induced apoptosis at concentrations of 1, 4, 8 mmol/L. Aspirin dose-dependently decreased the levels of COX-2 mRNA, COX-2 protein and nuclear NF-kappaB protein and increased the cytoplasmic IkappaB protein.

Conclusion:  We conclude that aspirin inhibits the proliferation of, and induced apoptosis in, the cultured TE-13 SCC cell line. These changes correlate with a reduction in COX-2 mRNA and protein expression, prostaglandin synthesis, an inhibition of NF-kappaB nuclear translocation, and an increase in cytoplasmic IkappaB. These results support the further investigation of the cyclooxygenase pathway in investigating the potential of aspirin and similar drugs in cancer prevention and therapy.

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